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首页> 外文期刊>Genetic Resources and Crop Evolution >Perennial kales: collection rationalization and genetic relatedness to other Brassica oleracea crop types
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Perennial kales: collection rationalization and genetic relatedness to other Brassica oleracea crop types

机译：多年生羽衣甘蓝：集合合理化和与其他芸苔属作物类型的遗传相关性

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摘要

Perennial kale is a rare leafy vegetable and forage crop that is mainly vegetatively propagated and therefore expensive to conserve ex situ. A genebank collection of 47 perennial kales and 34 reference samples from the main Brassica oleracea crop types were characterized with seven microsatellite markers in order to verify potential redundancies and to obtain more insight in the position of perennial kales within B. oleracea. Based on the obtained results and on data from previous studies, the collection was reduced with 49% to 24 perennial kales. Considering this level of reduction, it was estimated that the investments made for the final verification by microsatellite analysis are returned after only 4-year time. A principal coordinate plot clearly separated the perennial kales from the other crop types of B. oleracea, except in one case. This deviating accession of vegetatively preserved perennial kale clustered closely together with the single seed-preserved accession of perennial kale included in the study. These two accessions occupied an intermediate position between the group of vegetatively propagated perennial kales and the group of seed-propagated Brassica accessions, suggesting a hybridization background with another B. oleracea crop type. The microsatellite study demonstrated a close genetic relationship among the investigated perennial kales and their unique position within B. oleracea.
机译：多年生羽衣甘蓝是一种稀有的叶类蔬菜和饲料作物，主要通过营养繁殖，因此在异地保存很昂贵。利用七个微卫星标记对来自甘蓝菜主要农作物类型的47个多年生羽衣甘蓝和34个参考样品的种质库集合进行了表征，以验证潜在的冗余并获得对多年生羽衣甘蓝在B. oleracea中的位置的更多了解。根据获得的结果和以前的研究数据，该品种的多年生羽衣甘蓝减少了49％至24。考虑到这一下降水平，据估计，仅用了4年的时间就可以收回通过微卫星分析进行最终核查的投资。一个主坐标图清楚地将多年生羽衣甘蓝与油菜双歧杆菌的其他作物类型分开，除了一种情况。营养性保存的多年生羽衣甘蓝的这种偏离的加入与研究中包括的多年生羽衣甘蓝的单个种子保存的加入紧密地聚集在一起。这两个种在营养繁殖的多年生羽衣甘蓝组和种子繁殖的芸苔属组之间处于中间位置，这表明与另一种油菜芽孢杆菌的杂交背景。微卫星研究表明，所研究的多年生羽衣甘蓝与其在油菜双歧杆菌中的独特位置之间存在密切的遗传关系。

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