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首页> 外文期刊>Genetic Resources and Crop Evolution >Evaluation of genetic diversity of bread wheat landraces from Pakistan by AFLP and implications for a future collection strategy
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Evaluation of genetic diversity of bread wheat landraces from Pakistan by AFLP and implications for a future collection strategy

机译：通过AFLP评估巴基斯坦面包小麦地方品种的遗传多样性及其对未来收集策略的影响

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摘要

We used amplified-fragment-length polymorphism (AFLP) markers to evaluate genetic variation in a set of bread wheat (Triticum aestivum L.) landraces and improved materials. Landraces collected from different geographic and agro-ecological zones in Pakistan in 1987, 1989 and 1991 were separated into two groups based on their geographic origins: northern (Himalaya) and south-western (Balochistan) Pakistan. Six AFLP primer combinations detected 453 AFLP markers in the 43 landrace accessions and four high-yield varieties (HYVs). Of these, 225 (49.67%) were rare (shared with < 5% of all accessions). Among these rare alleles, 23 (10.22%) were common in the Himalaya (shared with > 10% of accessions collected there) but were not found in Balochistan. We conclude that there is a higher probability of collecting rare alleles at overall, but which are in contrast locally common ones in the Himalayan region. Gene diversity was 0.17 in the Himalayan group and 0.15 in the Balochistan group. Considerable genetic variability was found in both groups. Accessions from different agro-ecological zones were indistinguishable by cluster analysis, indicating intensive seed trading within the country. Cluster analysis indicated that the landraces and the HYVs are genetically distinct; suggesting that genetic erosion of wheat landraces has been unlikely taken in place. This study provides an example of how analysis of existing materials and data, can serve as a basis for future collection planning and conservation policies.
机译：我们使用扩增片段长度多态性（AFLP）标记来评估一组面包小麦（Triticum aestivum L.）地方品种和改良材料的遗传变异。 1987年，1989年和1991年从巴基斯坦不同地理和农业生态区收集的地方品种根据其地理起源分为两个组：巴基斯坦北部（喜马拉雅山）和西南部（巴洛克吉斯坦）。六个AFLP引物组合在43个地方品种和四个高产品种（HYV）中检测到453个AFLP标记。其中，有225种（占49.67％）是稀有的（占所有种质的5％以下）。在这些稀有等位基因中，有23个（占10.22％）在喜马拉雅山很常见（在那里收集到的种质占10％以上），但在Bal路支省却没有。我们得出的结论是，总体上收集稀有等位基因的可能性更高，但是与之相反，在喜马拉雅地区则是本地常见的等位基因。喜马拉雅组的基因多样性为0.17，the路支省的基因多样性为0.15。两组均发现相当大的遗传变异性。通过聚类分析无法区分来自不同农业生态区的种质，表明该国内部的种子交易密集。聚类分析表明，地方品种和HYV在遗传上是不同的。这表明小麦地方品种的遗传侵蚀不太可能发生。这项研究提供了一个示例，说明如何对现有材料和数据进行分析，可以作为将来收集计划和保护政策的基础。

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