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Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O. Kuntze, in Kyoto, Japan

机译:日本京都茶山茶(L.)O. Kuntze的当地地方茶种群内部和之中的微卫星变异性

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摘要

Genetic variability within and among eight landrace populations of tea (Camellia sinensis (L.) O. Kuntze) located in southern Kyoto, Japan, was surveyed with six microsatellite markers. The average number of alleles per locus was 3.83 to 4.67 for landrace populations, whereas the corresponding value among modern cultivars and breeding lines was 6.63. Expected heterozygosity values averaged over loci within landrace populations ranged from 0.498 to 0.723. A similar level of variation, 0.682, was observed for cultivars and breeding lines. High fixation index values (0.177–0.417) for each population are consistent with biparental inbreeding within the population. Genetic differentiation among local populations was extremely low with F ST = 0.062, although AMOVA revealed significant differentiation among landrace populations. We propose that these populations share a common ancestral gene pool and that some degree of artificial selection within each population has been performed by local farmers. Neighbor-joining analysis revealed that genetic relationships among populations reflect geographical location of populations. This might result from more frequent genetic exchange by nearby farmers.
机译:利用六个微卫星标记,对位于日本京都南部的八个茶(茶树(Camellia sinensis(L.)O. Kuntze))的内部和之中的遗传变异进行了调查。对于地方品种,每个基因座的平均等位基因数量为3.83至4.67,而在现代品种和育种系中,其对应值为6.63。在地方种群中,基因座的预期杂合度平均值为0.498至0.723。品种和育种系的变异水平相似,为0.682。每个种群的高固视指数值(0.177–0.417)与种群内的双亲近交是一致的。尽管AMOVA揭示了地方种群之间的显着差异,但当地种群之间的遗传分化极低,F ST = 0.062。我们建议这些种群共享一个共同的祖先基因库,并且每个种群中的某种程度的人工选择已由当地农民进行。邻居结合分析显示,种群之间的遗传关系反映了种群的地理位置。这可能是由于附近农民的基因交换更加频繁。

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