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Genetic Diversity in Barley (Hordeum vulgare L. ssp. vulgare) Landraces from Uttaranchal Himalaya of India

机译:印度Uttaranchal喜马拉雅山大麦(Hordeum vulgare L. ssp。vulgare)地方品种的遗传多样性

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In the high altitude areas of western Himalaya, barley is a crop of marginal, low input drought stressed environments. The landraces grown in these areas are favoured for their quality, both as grain and straw. However, area under the naked barley landraces, during the last three to four decades, has declined considerably and their ex situ and in situ conservation requires attention. Morphological and RAPD descriptors of a collection of 70 landraces from the higher Himalayan ranges of Uttaranchal state were used to analyse levels of genetic diversity. A wide range of variation was recorded for various morphological characters in univariate analysis. The multivariate analysis based on six quantitative traits distinguished accessions from different geographical areas in the region but failed to separate naked from covered barleys. Clustering based on qualitative traits, however, clearly distinguished naked and covered forms. RAPD profiles efficiently differentiated naked barleys from covered forms, but could not differentiate between oriental and occidental covered forms. A set of 11 predominant landraces were subjected to detailed population genetic analysis. More diversity was observed in covered barleys than the naked forms, both for morphological and RAPD markers. The low diversity in naked barley populations was attributed to either genetic drift or to a founder effect, while the high diversity in covered barley populations was attributed to their large-scale cultivation for animal feed and brewing purposes. Allelic combinations were not randomly distributed, as a geographic trend was closely related to covered and naked barleys. Since naked barleys are poorly represented in the national collections, a systematic collection from the entire region is suggested. The genetic differences between covered and naked barleys may be relevant to breeding programmes since the variability created through hybridisation between the contrasting forms could be exploited.
机译:在喜马拉雅山西部的高海拔地区,大麦是边缘,低投入干旱胁迫环境的一种作物。这些地区种植的地方品种以谷物和稻草的品质而受到青睐。然而,在过去的三到四十年中,裸露的大麦地方品种的面积已大大减少,它们的非原生境和原生境保存需要引起注意。从形态分布特征和RAPD描述子中收集了来自北阿坎恰尔邦喜马拉雅山脉较高范围的70个地方品种的信息,用于分析遗传多样性的水平。在单变量分析中记录了各种形态特征的广泛变化。基于六种数量性状的多变量分析区分了该地区不同地理区域的种质,但未能将裸麦与覆盖大麦区分开。但是,基于定性特征的聚类可以清楚地区分裸露和掩盖的形式。 RAPD谱有效地区分了裸大麦与被覆形式,但无法区分东方和西方被覆形式。对一组11个主要的地方品种进行了详细的种群遗传分析。在覆盖的大麦中,形态和RAPD标记均比裸露的大。裸大麦种群的低多样性归因于遗传漂移或奠基者效应,而覆盖大麦种群的高多样性归因于其大规模种植用于动物饲料和酿造目的。等位基因组合不是随机分布的,因为地理趋势与有盖大麦和裸露大麦密切相关。由于裸露的大麦在国家藏品中的代表性不高,因此建议从整个地区进行系统收集。有盖大麦和裸露大麦之间的遗传差异可能与育种计划有关,因为可以利用通过对比形式之间杂交产生的变异性。

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