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首页> 外文期刊>Genetic Resources and Crop Evolution >Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers
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Structure of genetic diversity among common bean (Phaseolus vulgaris L.) varieties of Mesoamerican and Andean origins using new developed microsatellite markers

机译：使用新开发的微卫星标记的中美洲和安第斯血统的普通豆（菜豆）品种遗传多样性结构

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摘要

A common bean genomic library was constructed using the ‘IAC-UNA’ variety enriched for (CT) and (GT) for microsatellite motifs. From 1,209 sequenced clones, 714 showed microsatellites distributed over 471 simple and 243 compound motifs. GA/CT and GT/CA were the most frequent motifs found among these sequences. A total of 123 microsatellites has been characterized. Out of these, 87 were polymorphic (73.7%), 33 monomorphic (26.8%), and 3 (2.4%) did not amplify at all. In a sample of 20 common bean materials selected from the Agronomic Institute Germplasm Bank, the number of alleles per locus varied 2–9, with an average of 2.82. The polymorphic information content (PIC) of each marker varied from 0.05 to 0.83, with a 0.45 average value. Cluster and principal coordinate analysis of the microsatellite data were consistent with the original assignment of the germplasm accessions into the Andean and Mesoamerican gene pools of common bean. Low polymorphism levels detected could be associated with the domestication process. These microsatellites could be a valuable resource for the bean community because of their use as new markers for genetic studies.
机译：使用富含“（CT）”和“（GT）”微卫星图案的“ IAC-UNA”变种构建了一个常见的豆类基因组库。在1,209个测序克隆中，有714个显示出微卫星分布在471个简单和243个复合基序上。 GA / CT和GT / CA是这些序列中最常见的基序。总共鉴定了123个微卫星。其中87个是多态的（73.7％），33个单态的（26.8％）和3个（2.4％）根本没有扩增。在从农艺研究所种质库中选出的20种常见豆类材料的样本中，每个基因座的等位基因数量在2–9之间变化，平均为2.82。每个标记的多态信息含量（PIC）在0.05到0.83之间变化，平均值为0.45。微卫星数据的聚类和主坐标分析与种质种质最初进入普通豆的安第斯和中美洲基因库的分配是一致的。检测到的低多态性水平可能与驯化过程有关。这些微卫星可能是豆类社区的宝贵资源，因为它们被用作遗传研究的新标记。

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