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首页> 外文期刊>Genetica >Genomic diversity of germinating scutellum specific gene P23k in barley and wheat
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Genomic diversity of germinating scutellum specific gene P23k in barley and wheat

机译：大麦和小麦中发芽盾片特异基因P23k的基因组多样性

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摘要

P23k is a 23 kDa protein involved in sugar translocation in the scutellum of germinating barley seeds. The present study was carried out to provide the genomic characterization for P23k gene in terms of copy number, chromosome mapping, genetic mapping and expression analysis in germinating sculletum in two major Triticeae crops, barley and wheat, and their relatives. Southern blotting showed that a variable copy number with different restriction fragment sizes was found among 15 Hordeum accessions, while low copy number were found to be conserved in 23 Triticum and 3 Aegilops accessions. Genetic and physical mapping study identified that the P23k gene is duplicated in wild and cultivated barley on chromosomes 1H, 2H, and 3H, and further tandem duplication on chromosomes 1H and 3H. In contrast, the wheat P23k is located on chromosome 3A of durum wheat and at the distal portion of the long arms of 3A and 3D chromosomes of bread wheat. Northern blotting showed remarkably high accumulation of P23k transcript in the germinating scutellum in cultivated and wild barley, whereas very few or no accumulation was detected in diploid, tetraploid, and hexaploid wheat accessions. The present study suggests a simple scenario where the ancestral P23k is encoded on the distal portion of an ancestral chromosome of homoeologous chromosome 3. Beside of polyploidy, dispersed and tandem duplications could trigger generation of the P23k family in the Hordeum lineage, while an ancestral P23k has been conserved in homoeologous 3A and 3D chromosomes in the wheat lineage. Keywords P23k - Barley seed - Germinating scutellum - Gene duplication
机译：P23k是一种23 kDa的蛋白质，参与发芽的大麦种子盾片中的糖转运。进行本研究以提供P23k基因的基因组学特性，包括大麦和小麦及其近亲的两种主要麦类作物的发芽雕塑中的拷贝数，染色体作图，遗传作图和表达分析。 Southern印迹表明，在15个大麦属种中发现了具有不同限制片段大小的可变拷贝数，而在23个小麦和3个Aegilops种中发现了低拷贝数。遗传和物理作图研究确定，P23k基因在1H，2H和3H染色体的野生和栽培大麦中复制，并在1H和3H染色体上进一步串联复制。相反，小麦P23k位于硬粒小麦的3A染色体上，并且位于面包小麦的3A和3D染色体长臂的末端。 Northern印迹表明，在栽培和野生大麦中发芽的盾片中P23k转录物的积累非常高，而在二倍体，四倍体和六倍体小麦种中却很少或没有积累。本研究提出了一个简单的方案，其中祖先P23k编码在同源染色体3的祖先染色体的远端。除了多倍体外，分散和串联重复可能触发大麦谱系中P23k家族的产生，而祖先P23k已经在小麦谱系中的同源3A和3D染色体中保守了。 P23k-大麦种子-盾片发芽-基因复制

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