• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetica >Patterns of DNA sequence variation at candidate gene loci in black poplar (Populus nigra L.) as revealed by single nucleotide polymorphisms
【24h】

Patterns of DNA sequence variation at candidate gene loci in black poplar (Populus nigra L.) as revealed by single nucleotide polymorphisms

机译：单核苷酸多态性揭示黑杨（Populus nigra L.）候选基因位点的DNA序列变异模式

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Black poplar (Populus nigra L.) is an economically and ecologically important tree species and an ideal organism for studies of genetic variation. In the present work, we use a candidate gene approach to infer the patterns of DNA variation in natural populations of this species. A total of 312 single nucleotide polymorphisms (SNPs) are found among 8,056 bp sequenced from nine drought-adaptation and photosynthesis-related gene loci. The median SNP frequency is one site per 26 bp. The average nucleotide diversity is calculated to be θW = 0.01074 and πT = 0.00702, higher values than those observed in P. tremula, P. trichocarpa and most conifer species. Tests of neutrality for each gene reveal a general excess of low-frequency mutations, a greater number of haplotypes than expected and an excess of high-frequency derived variants in P. nigra, which is consistent with previous findings that genetic hitchhiking has occurred in this species. Linkage disequilibrium is low, decaying rapidly from 0.45 to 0.20 or less within a distance of 300 bp, although the declines of r 2 are variable among different loci. This is similar to the rate of decay reported in most other tree species. Our dataset is expected to enhance understanding of how evolutionary forces shape genetic variation, and it will contribute to molecular breeding in black poplar. Keywords Single nucleotide polymorphism (SNP) - Populus nigra - Candidate gene - Nucleotide diversity - Linkage disequilibrium
机译：黑杨（Populus nigra L.）是一种经济上和生态上重要的树种，也是研究遗传变异的理想生物。在目前的工作中，我们使用候选基因方法来推断该物种自然种群中DNA变异的模式。在9个干旱适应和光合作用相关基因位点的8,056 bp序列中，共发现312个单核苷酸多态性（SNP）。中位SNP频率是每26 bp一个位点。计算得出的平均核苷酸多样性为θ W = 0.01074和π T = 0.00702，这比在颤栗木，毛果木和大多数针叶树种中观察到的值高。每个基因的中立性测试显示，黑假单胞菌中普遍存在低频突变过多，单倍型数量多于预期以及黑斑病中高频衍生变异过多的现象，这与以前的研究发现遗传搭便车相一致。种类。连锁不平衡很低，在300 bp的距离内从0.45迅速衰减至0.20或更小，尽管r 2 的下降在不同位点之间是可变的。这与大多数其他树种中报道的腐烂率相似。我们的数据集有望增进人们对进化力如何影响遗传变异的理解，并将有助于黑杨的分子育种。关键词单核苷酸多态性（SNP）-黑杨-候选基因-核苷酸多样性-连锁不平衡

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号