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首页> 外文期刊>Genetica >Characterization of mitochondrial control region, two intergenic spacers and tRNAs of Zaprionus indianus (Diptera: Drosophilidae)
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Characterization of mitochondrial control region, two intergenic spacers and tRNAs of Zaprionus indianus (Diptera: Drosophilidae)

机译：Zaprionus indianus（Diptera：Drosophilidae）的线粒体控制区，两个基因间隔区和tRNA的特征

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摘要

The control region in insects is the major noncoding region in animal mitochondrial DNA (mtDNA), and is responsible for a large part of the variation in the DNA sequence and size of the genome of this organelle. In this study, the mtDNA control region, two intergenic spacers and tRNA genes of a Zaprionus indianus strain were cloned, sequenced and compared with other Drosophila species. The overall A+T content in the Z. indianus control region is 94.3%, and a comparison with other Drosophila species demonstrated that the most conserved region appears to be the 420 base pairs nearest to the tRNAile, similar to the findings of other authors. We also describe conserved sequence blocks, including a poly-T involved in the replication process of Drosophila mtDNA; a putative secondary structure also involved in the replication process and repeated sequences. tRNAile sequence demonstrated the greatest variability when the tRNA sequences of species were compared. Keywords Zaprionus indianus - Drosophila - Control region - tRNAs - Secondary structure
机译：昆虫的控制区是动物线粒体DNA（mtDNA）中的主要非编码区，并负责该细胞器DNA序列和基因组大小的很大一部分变异。在这项研究中，Zaprionus indianus菌株的mtDNA控制区，两个基因间隔区和tRNA基因被克隆，测序并与其他果蝇物种进行了比较。印第安那虫控制区的总A + T含量为94.3％，与其他果蝇物种的比较表明，最保守的区域似乎是最接近tRNA ile 的420个碱基对，与其他作者的发现相似。我们还描述了保守序列块，包括果蝇mtDNA复制过程中涉及的poly-T；推定的二级结构也参与复制过程和重复序列。比较物种的tRNA序列时，tRNA ile 序列表现出最大的变异性。关键词Zaprionus indianus-果蝇-控制区-tRNAs-二级结构

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