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首页> 外文期刊>Genetica >Duplicated Clock genes with unique polyglutamine domains provide evidence for nonhomologous recombination in Chinook salmon (Oncorhynchus tshawytscha)
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Duplicated Clock genes with unique polyglutamine domains provide evidence for nonhomologous recombination in Chinook salmon (Oncorhynchus tshawytscha)

机译：具有独特聚谷氨酰胺结构域的重复Clock基因为奇努克鲑鱼（Oncorhynchus tshawytscha）的非同源重组提供了证据

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摘要

Circadian rhythms underlie diverse life functions ranging from cellular activities to behavior. Multiple clock genes play a central role in the generation of these rhythms. We partially characterized two copies of the Clock gene from Chinook salmon (Oncorhynchus tshawytscha), OtsClock1a and OtsClock1b. The 6,460 bp OtsClock1a sequence contains 16 exons, 15 introns and encompasses three highly conserved domains indicating it is a novel member of the bHLH-PAS superfamily of transcription factors. The second copy, OtsClock1b, consists of five exons and five introns spanning 1,945 bp. A polyglutamine repeat motif (PolyQ), characteristic of a majority of CLOCK proteins, is present in both OTSCLOCK1a and OTSCLOCK1b. However, the Chinook PolyQ domains are uniquely positioned inside the gene. Interestingly, a 1,200 bp non-coding segment located downstream of the OtsClock1a PolyQ domain is absent from OtsClock1b. This insertion/deletion is 91% similar to the Salmo salar Transferrin gene. A phylogenetic analysis of 11 CLOCK proteins shows that OtsClock1a and OtsClock1b are paralogs which likely arose subsequent to the salmonid genome-wide duplication event. Ultimately, the Chinook salmon Clock genes are key components to our understanding the genetic mechanisms underlying temporally regulated life history traits in Pacific salmonids.
机译：昼夜节律是从细胞活动到行为的多种生命功能的基础。多个时钟基因在这些节奏的产生中起着核心作用。我们部分表征了来自奇努克鲑鱼（Oncorhynchus tshawytscha）的Clock基因的两个副本，OtsClock1a和OtsClock1b。该6,460 bp的OtsClock1a序列包含16个外显子，15个内含子，并包含三个高度保守的结构域，表明它是转录因子bHLH-PAS超家族的新成员。第二个副本OtsClock1b由五个外显子和五个内含子组成，跨度为1,945 bp。 OTSCLOCK1a和OTSCLOCK1b中都存在大多数谷氨酸（CLOCK）蛋白特有的聚谷氨酰胺重复基序（PolyQ）。但是，奇努克（Chinook）PolyQ域在基因内部唯一定位。有趣的是，OtsClock1b没有位于OtsClock1a PolyQ域下游的1200 bp非编码段。该插入/缺失与Salmo salar转铁蛋白基因相似，为91％。对11个CLOCK蛋白的系统进化分析表明，OtsClock1a和OtsClock1b是可能在鲑鱼全基因组复制事件后出现的旁系同源物。最终，奇努克鲑鱼时钟基因是我们了解太平洋鲑鱼暂时调控生活史特征的遗传机制的关键组成部分。

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