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首页> 外文期刊>Genetics >The multiple-wing-hairs Gene Encodes a Novel GBD–FH3 Domain-Containing Protein That Functions Both Prior to and After Wing Hair Initiation
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The multiple-wing-hairs Gene Encodes a Novel GBD–FH3 Domain-Containing Protein That Functions Both Prior to and After Wing Hair Initiation

机译：多翼毛基因编码一种新型的含GBD–FH3结构域的蛋白，该蛋白在翼发开始之前和之后均起作用

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The frizzled signaling/signal transduction pathway controls planar cell polarity (PCP) in bothnvertebrates and invertebrates. Epistasis experiments argue that in the Drosophila epidermis multiple wingnhairs (mwh) acts as a downstream component of the pathway. The PCP proteins accumulate asymmetricallynin pupal wing cells where they are thought to form distinct protein complexes. One is located on the distalnside of wing cells and a second on the proximal side. This asymmetric protein accumulation is thought tonlead to the activation of the cytoskeleton on the distal side, which in turn leads to each cell forming ansingle distally pointing hair. We identified mwh as CG13913, which encodes a novel G protein bindingndomain–formin homology 3 (GBD–FH3) domain protein. The Mwh protein accumulated on thenproximal side of wing cells prior to hair formation. Unlike planar polarity proteins such as Frizzled ornInturned, Mwh also accumulated in growing hairs. This suggested that mwh had two temporally separatenfunctions in wing development. Evidence for these two functions also came from temperature-shiftnexperiments with a temperature-sensitive allele. Overexpression of Mwh inhibited hair initiation, thusnMwh acts as a negative regulator of the cytoskeleton. Our data argued early proximal Mwh accumulationnrestricts hair initiation to the distal side of wing cells and the later hair accumulation of Mwh prevents thenformation of ectopic secondary hairs. This later function appears to be a feedback mechanism that limitsncytoskeleton activation to ensure a single hair is formed.
机译：卷曲的信号/信号转导途径控制着无脊椎动物和无脊椎动物中的平面细胞极性（PCP）。上位性实验证明，在果蝇表皮中，多个翼毛（mwh）充当该途径的下游成分。 PCP蛋白在a翼细胞中不对称地积累，据认为它们会形成独特的蛋白复合物。一个位于机翼细胞的远端，第二个位于近端。这种不对称蛋白质的积累被认为会导致远端细胞骨架的激活，进而导致每个细胞形成单个指向远端的毛发。我们将mwh识别为CG13913，它编码一种新的G蛋白结缔结构域-结构蛋白同源性3（GBD-FH3）域蛋白。在头发形成之前，Mwh蛋白积累在翼细胞的近端。与平面极性蛋白质（如卷曲的ornInturned）不同，Mwh还在不断生长的头发中积累。这表明mwh在机翼发育中具有两个暂时的功能。这两个功能的证据也来自对温度敏感的等位基因的温度漂移实验。 Mwh的过表达抑制了头发的萌发，因此nMwh充当了细胞骨架的负调节剂。我们的数据认为，早期的近端Mwh积累限制了毛发向翼细胞远端的传播，而后期的Mwh积累则阻止了异位继发的形成。后来的功能似乎是一种反馈机制，它限制了细胞骨架的激活，以确保形成一根头发。

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