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Bacterial Community Shift in Two Sectors of a Tannery Plant and its Cr (VI) Removing Potential

机译:制革厂两个部门的细菌群落迁移及其去除六价铬的潜力

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The bacterial community, analyzed by both 16S rRNA gene-based clone library and cultivation methods, changed in two different sectors of a tannery plant. Ten different bacterial phyla were detected through the clone library. The bacterial community of the tanning waste consisted of Gammaproteobacteria (34.8%), Bacteroidetes (20.9%), Betaproteobacteria (12.8%), Alphaproteobacteria (7.0%), Firmicutes (7.0%), Deinococcus-Thermus (7%), Planctomycetes (4.7%), Verrucomicrobia (2.3%), Epsilonproteobacteria (1.2%), Spirochaetes (1.2%), and that of the settling tank sludge consisted of Firmicutes (38.8%), Betaproteobacteria (27.1%), Gammaproteobacteria (11.8%), Bacteroidetes (9.4%), Alphaproteobacteria (4.7%), Deltaproteobacteria (3.5%), Actinobacteria (2.4%), Synergistes (1.2%). The tanning waste accommodated higher biodiversity than the downstream primary settling tank did. Cultivation conditions seriously influenced the culturability and isolation of bacterial strains: different levels of nutrients and Cr (VI) and temperatures resulted in isolation of different bacterial strains, which showed different removal of Cr (VI) (from 1.2% to 99.1%). In addition, supplementation of nutritious LB medium enhanced the Cr (VI) culturability of bacterial isolates. Several bacteria detected as operational taxonomy units (OTUs) by culture-independent analysis were isolated, yet many OTUs did not have their relative isolates. Among the isolates, Pseudochrobactrum, Shewanella, Marinobacter, Microbacterium, Psychrobacter and Agrococcus strains showed considerable Cr (VI) removal efficiencies and good potentials for Cr (VI) pollution treatment.View full textDownload full textKeywordstannery waste, bacterial community, clone library analysis, bacterial cultivation, Cr (VI) removalRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/01490451.2011.558562
机译:通过基于16S rRNA基因的克隆文库和培养方法分析的细菌群落在制革厂的两个不同部门发生了变化。通过克隆文库检测到十种不同的细菌门。制革废料的细菌群落包括γ-变形杆菌(34.8%),拟杆菌(20.9%),β-变形细菌(12.8%),α-变形细菌(7.0%),硬毛菌(7.0%),脱球菌-栖热菌(7%),扁平菌(4.7) %),Verrucomicrobia(2.3%),Epsilonproteobacteria(1.2%),Spirochaetes(1.2%)和沉淀池污泥中的包括Firmicutes(38.8%),Betaproteobacteria(27.1%),γ-proteobacteria(11.8%),Bacteroidetes( 9.4%),α-变形杆菌(4.7%),δ-变形杆菌(3.5%),放线菌(2.4%),协同菌(1.2%)。与下游主要沉淀池相比,鞣制废物具有更高的生物多样性。耕种条件严重影响了细菌菌株的可培养性和分离性:不同水平的养分和六价铬和温度导致不同细菌菌株的分离,这表明六价铬的去除率不同(从1.2%到99.1%)。此外,补充营养丰富的LB培养基可增强细菌分离株的Cr(VI)可培养性。分离了通过培养无关分析检测为操作分类单位(OTU)的几种细菌,但许多OTU没有其相对分离株。在这些菌株中,假单孢菌,希瓦氏菌,马氏杆菌,微细菌,精神杆菌和农杆菌菌株显示出相当高的Cr(VI)去除效率,并且具有很好的Cr(VI)污染处理潜力。查看全文下载全文关键词废渣,细菌群落,克隆文库分析,细菌栽培,六价铬的去除相关的变量addthis_config = {ui_cobrand:“泰勒和弗朗西斯在线”,servicescompact:“ citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,更多”,pubid:“ ra -4dff56cd6bb1830b“};添加到候选列表链接永久链接http://dx.doi.org/10.1080/01490451.2011.558562

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