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首页> 外文期刊>Geomicrobiology Journal >Bacterial Community Composition and Diversity in Methane Charged Sediments Revealed by Multitag Pyrosequencing
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Bacterial Community Composition and Diversity in Methane Charged Sediments Revealed by Multitag Pyrosequencing

机译：多标签焦磷酸测序揭示甲烷带电沉积物中细菌群落组成和多样性

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摘要

Bacterial diversity in sediments obtained along the Chilean margin from areas containing methane seeps, and a hydrate mound were explored by cloning and sequencing and multitag pyrosequencing (MTPS). These libraries were statistically compared to determine the robustness of taxonomic assignment derived from multiplexed pyrosequencing strategies targeting variable regions V1 and V2 of the small subunit rRNA gene for environmental studies. There was no statistical difference in the composition of the libraries, thus, MTPS was utilized to describe diversity in three geochemical zones in these environments. Unidentified Cyanobacteria isolates were abundant in the sulfate reduction zone (SRZ), Deltaproteobacteria were concentrated at the sulfate methane transition zone (SMTZ) and Chloroflexi/GNS dominated methanogenesis zone (MGZ). Although there was variation among specific groups, communities in the SRZ and MGZ did not differ significantly. However, the community dominated by Deltaproteobacteria differentiates the SMTZ from the other zones. Supplemental materials are available for this article. Go to the publisher's online edition of Geomicrobiology Journal to view the free supplemental file.View full textDownload full textKeywordsAOM, bacteria, Chilean margin, methane, multi-tag, pyrosequencing, sulfateRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/01490451.2011.559305
机译：通过克隆和测序以及多标签焦磷酸测序（MTPS）探索了智利边缘沿含甲烷渗漏和水合物丘陵地区沉积物中细菌的多样性。对这些文库进行统计比较，以确定针对小亚基rRNA基因可变区V1和V2的多重焦磷酸测序策略衍生的分类学分类的鲁棒性，以进行环境研究。库的组成没有统计学差异，因此，MTPS用于描述这些环境中三个地球化学区的多样性。未鉴定的蓝细菌分离物在硫酸盐还原区（SRZ）中丰富，Deltaproteobacteria细菌集中在硫酸盐甲烷过渡区（SMTZ）和Chloroflexi / GNS占主导的产甲烷区（MGZ）。尽管特定人群之间存在差异，但SRZ和MGZ中的社区并没有显着差异。但是，以三角洲变形杆菌为主导的社区将SMTZ与其他地区区分开来。补充材料可用于本文。转到出版商的《地球微生物学期刊》在线版本，以查看免费的补充文件。 ：：“ citeulike，netvibes，twitter，technorati，美味，linkedin，facebook，stumbleupon，digg，google，更多”，pubid：“ ra-4dff56cd6bb1830b”};添加到候选列表链接永久链接http://dx.doi.org/10.1080/01490451.2011.559305

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