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首页> 外文期刊>Deutsche Molkerei Zeitung >In vitro cytotoxic activity of probiotic bacterial cell extracts against Caco-2 and HRT-18 colorectal cancer cells
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In vitro cytotoxic activity of probiotic bacterial cell extracts against Caco-2 and HRT-18 colorectal cancer cells

机译:益生菌细菌细胞提取物对Caco-2和HRT-18大肠癌细胞的体外细胞毒活性

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摘要

This research aimed at Screening the anti-cancer activity of cell extracts of forty potential probiotic bacterial isolates against 2 colorectal cancer (CRC) cell lines (namely, Caco-2 and HRT-18), and vero cells using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyl-tetrazolium bromide (MU) and Trypan Blue assays (TBE). Results demonstrated that 2 isolates (Lactobacillus acidophilus LA102 and Lactobacillus casei LC232) showed pronounced cytotoxic ac-tivities, with proliferation inhibition of 37% and 68.5% of LA102, and 48% and 45.7% of LC232 against Caco-2 and HRT-18, res-pectively, at a concentration of 100 tg extract/mi. The IC50 values of the cytotoxic activity were 1.6 and 2.5 ig/ml of LA102, and 15.4 and 6.2 ig/ml of LC232 against Caco-2 and HRT-18, respec-tively. At the same time, results showed that LA102 and LC232 isolates had no cytotoxic effect on the normal vero cells. Even though these observations raise the prospects of using these pro-biotic isolates for possible cancer prevention and even treatment, yet further investigation is needed to ascertain their potential to prevent in vivo human CRC.
机译:这项研究旨在使用3-(4,5)筛选40种潜在益生菌菌株的细胞提取物对2种结直肠癌(CRC)细胞系(即Caco-2和HRT-18)和vero细胞的抗癌活性。 -二甲基噻唑基-2)-2、5-二苯基溴化四唑鎓(MU)和锥虫蓝测定(TBE)。结果表明,2种分离株(嗜酸乳杆菌LA102和干酪乳杆菌LC232)表现出明显的细胞毒活性,对LA102的抑制分别为37%和68.5%,对Caco-2和HRT-18的抑制分别为48%和45.7%。分别为100吨提取物/毫升的浓度。相对于Caco-2和HRT-18,LA102的细胞毒性活性的IC50值为1.6和2.5 ig / ml,LC232的为15.4和6.2 ig / ml。同时,结果显示,LA102和LC232分离株对正常vero细胞没有细胞毒性作用。即使这些观察结果提出了将这些益生菌分离物用于可能的癌症预防甚至治疗的前景,但仍需要进一步研究以确定其预防体内人CRC的潜力。

著录项

  • 来源
    《Deutsche Molkerei Zeitung》 |2017年第5期|1-5|共5页
  • 作者单位

    Food Microbiology and Safety Laboratory, Department of Nutrition and Food Processing, Faculty of Agricultural Technology-Balq'a Applied University, P.O. Box 19117, Salt, Jordan;

    Department of Biotechnology, Faculty of Agriculturural Technology- Balq'a Applied University, P.O. Box 19117, Salt, Jordan;

    Department of Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan;

    Department of Biopharmaceutics and Clinical Pharmacy. Faculty of Pharmacy, The University of Jordan. Amman 11942, Jordan;

    Food Microbiology and Safety Laboratory, Department of Nutrition and Food Processing, Faculty of Agricultural Technology-Balq'a Applied University, P.O. Box 19117, Salt, Jordan;

    Food Microbiology and Safety Laboratory, Department of Nutrition and Food Processing, Faculty of Agricultural Technology-Balq'a Applied University, P.O. Box 19117, Salt, Jordan;

    Food Microbiology and Safety Laboratory, Department of Nutrition and Food Processing, Faculty of Agricultural Technology-Balq'a Applied University, P.O. Box 19117, Salt, Jordan;

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  • 原文格式 PDF
  • 正文语种 ger
  • 中图分类
  • 关键词

    Probio tic; colorectal cancer; Lactic acid bacteria, Lac-tobacillus; MTT; cytotoxicity;

    机译:益生元;大肠癌;乳酸菌;乳杆菌;MTT;细胞毒性;

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