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首页> 外文期刊>Deutsche Zeitschrift fur Sportmedisin >DNA-Schäden in Leukocyten nach einem Triathlonwettkampf über die Olympische Distanz
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DNA-Schäden in Leukocyten nach einem Triathlonwettkampf über die Olympische Distanz

机译：铁人三项比赛在奥林匹克距离上对白细胞的DNA损伤

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The connection between intensive exercise and the induction of radical-mediated cellular damage is known for a long time. An association between anaerobic exercise and the induction of damage on the DNA level has been shown recently by means of the alkaline comet assay (single cell gel electrophoresis), a sensitive test for the detection of primary DNA damage. The aim of the present study was: (1.) to measure the extent of DNA damage after a triathlon competition and to follow the changes over time, (2.) to investigate, whether the DNA damage detected by the comet assay leads to damage on chromosomal level. To answer this question we used the micronucle-us assay, which is well established in human biomonitoring. Venous blood of six triathle-tes was taken prior to the competition (1,5 km S, 40 km B, 10 km R) and every 24 hrs up to day 5 after the the triathlon, and investigated in the comet and micronucleus assay. A strong increase of damaged DNA was seen in the comet assay in comparison to the pre-exercise level indicated by an elevated number of DNA fragments migrating out of the nucleus during electrophoresis (tail moment, TM). The maximum of DNA damage was observed 3 days after the competition (mean TM 2.37 at day 3 vs. 0.02 before the competition). Even at day 5 after the exercise the TM-values have still been significantly higher than the control values (TM 0.23 vs. 0.02). Despite the distinct effects shown in the comet assay, intensive exercise did not lead to an increased number of micronuclei in the leucocytes of the triathletes (micronucleus frequency before: 0.68%, day 2:0.73%, day 4:0.73%). Thus the massive primary DNA damage revealed in the comet assay does not seem to manifest in damage on chromosomal level. The causes and the meaning of the effects observed in the comet assay remain to be clarified.
机译：长期以来，剧烈运动与诱发自由基介导的细胞损伤之间的联系是众所周知的。最近已经通过碱性彗星试验（单细胞凝胶电泳）证明了无氧运动与DNA水平损伤的诱导之间的联系，这是一种检测初级DNA损伤的灵敏测试。本研究的目的是：（1.）测量铁人三项比赛后DNA损伤的程度并跟踪随时间的变化，（2。）调查彗星试验检测到的DNA损伤是否导致了损伤在染色体水平上。为了回答这个问题，我们使用了微核-us分析法，该方法在人类生物监测中已得到很好的确立。比赛前（1.5 km，40 km B，10 km R）在铁人三项赛之后的第5天之前，每24小时采集六个铁人三项的静脉血，并在彗星和微核试验中进行调查。与运动前的水平相比，在彗星试验中观察到受损DNA的强烈增加，这是由电泳过程中从尾核中迁移出的DNA片段数量增加所指示的（尾矩，TM）。比赛后3天观察到最大的DNA损伤（第3天的平均TM 2.37比比赛前的0.02）。即使在运动后的第5天，TM值仍显着高于对照值（TM 0.23对0.02）。尽管在彗星试验中显示出明显的作用，但是剧烈运动并没有导致铁人三项运动员的白细胞中的微核数量增加（之前的微核频率：0.68％，第2天：0.73％，第4天：0.73％）。因此，彗星试验中揭示的大量初级DNA损伤似乎并未表现出染色体水平的损伤。在彗星试验中观察到的影响的原因和含义还有待澄清。

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