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首页> 外文期刊>Harmful Algae >A novel portable filtration system for sampling and concentration of microorganisms: Demonstration on marine microalgae with subsequent quantification using IC-NASBA
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A novel portable filtration system for sampling and concentration of microorganisms: Demonstration on marine microalgae with subsequent quantification using IC-NASBA

机译：一种用于微生物采样和浓缩的新型便携式过滤系统：在海洋微藻上进行演示并随后使用IC-NASBA进行定量

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This paper presents a novel portable sample filtration/concentration system, designed for use on samples of microorganisms with very low cell concentrations and large volumes, such as water-borne parasites, pathogens associated with faecal matter, or toxic phytoplankton. The example application used for demonstration was the in-field collection and concentration of microalgae from seawater samples. This type of organism is responsible for Harmful Algal Blooms (HABs), an example of which is commonly referred to as "red tides", which are typically the result of rapid proliferation and high biomass accumulation of harmful microalgal species in the water column or at the sea surface. For instance, Karenia brevis red tides are the cause of aquatic organism mortality and persistent blooms may cause widespread die-offs of populations of other organisms including vertebrates. In order to respond to, and adequately manage HABs, monitoring of toxic microalgae is required and large-volume sample concentrators would be a useful tool for in situ monitoring of HABs. The filtering system presented in this work enables consistent sample collection and concentration from 1 L to 1 mL in five minutes, allowing for subsequent benchtop sample extraction and analysis using molecular methods such as NASBA and IC-NASBA. The microalga Tetraselmis suecica was successfully detected at concentrations ranging from 2 x 10(5) cells/L to 20 cells/L. Karenia brevis was also detected and quantified at concentrations between 10 cells/L and 10(6) cells/L. Further analysis showed that the filter system, which concentrates cells from very large volumes with consequently more reliable sampling, produced samples that were more consistent than the independent non-filtered samples (benchtop controls), with a logarithmic dependency on increasing cell numbers. This filtering system provides simple, rapid, and consistent sample collection and concentration for further analysis, and could be applied to a wide range of different samples and target organisms in situations lacking laboratories. (C) 2018 Published by Elsevier B.V.
机译：本文介绍了一种新颖的便携式样品过滤/浓缩系统，该系统设计用于细胞浓度极低且体积较大的微生物样品，例如水生寄生虫，与粪便有关的病原体或有毒浮游植物。用于演示的示例应用是从海水样品中现场收集和浓缩微藻。这种生物负责有害藻华（HABs），其中一个例子通常称为“赤潮”，这通常是有害的微藻物种在水柱或水域快速繁殖和高生物量积累的结果。海面。例如，短小克雷伯氏红潮是水生生物死亡的原因，持续的开花可能导致包括脊椎动物在内的其他生物种群的广泛死亡。为了对HAB做出反应并进行适当管理，需要监测有毒微藻，并且大体积的样品浓缩器将是对HAB进行原位监测的有用工具。这项工作中介绍的过滤系统能够在五分钟内从1 L到1 mL进行一致的样品收集和浓缩，从而允许随后使用NASBA和IC-NASBA等分子方法进行台式样品的提取和分析。在2 x 10（5）细胞/ L至20细胞/ L的浓度范围内成功检测到微藻Teecselmis suecica。还检测到了短小卡伦氏菌（Karenia brevis），并在10细胞/ L和10（6）细胞/ L之间的浓度下对其进行了定量。进一步的分析表明，该过滤器系统可以从非常大的体积中浓缩细胞，从而获得更可靠的采样，所产生的样品比独立的未过滤样品（台式对照）更加一致，并且对数依赖于增加的细胞数。该过滤系统可提供简单，快速且一致的样品收集和浓缩以进行进一步分析，并且在缺乏实验室的情况下，可应用于各种不同的样品和目标生物。 （C）2018由Elsevier B.V.发布

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