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首页> 外文期刊>Harmful Algae >Variability of paralytic shellfish toxin occurrence and profiles in bivalve molluscs from Great Britain from official control monitoring as determined by pre-column oxidation liquid chromatography and implications for applying immunochemical tests
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Variability of paralytic shellfish toxin occurrence and profiles in bivalve molluscs from Great Britain from official control monitoring as determined by pre-column oxidation liquid chromatography and implications for applying immunochemical tests

机译：通过柱前氧化液相色谱测定的官方对照监测显示，英国双壳贝类软体动物中麻痹性贝类毒素的发生和分布的变异性及其对免疫化学测试的意义

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摘要

As the official control monitoring laboratory in Great Britain for the analysis of marine biotoxins in shellfish, Cefas have for the past five years conducted routine monitoring for paralytic shellfish poisoning toxins (PST) using a non-animal alternative method to the mouse bioassay reference method; a refined version of the AOAC 2005.06 pre-column oxidation liquid chromatography method. Application of this instrumental methodology has enabled the generation of data not only on the occurrence and magnitude of PST events, but also the quantitation and assessment of different PST profiles. Since implementation of the method in 2008, results have shown huge variabilities in the occurrence of PSTs, with large spatial and temporal variabilities around the coastline. Mean PST profiles were not found to correlate either with total PST content of the shellfish, the year of sampling or with a few notable exceptions, the shellfish species. Toxin profiles were found to fall into one of four distinct profile types, with one relating solely to the exclusive presence of decarbamoyl toxins in surf clams. The other profile types contained variable proportions of gonyautoxins, N-sulfocarbamoyl toxins, neosaxitoxin and saxitoxin. While some indications of geographical repeatability were noted, this was not observed for all profile types. Consequently, the application of rapid immunochemical testing methods to end product testing would need to be considered carefully given the large differences in PST congener cross-reactivities.
机译：作为英国官方的监测贝类海洋生物毒素的对照监控实验室，Cefas在过去五年中采用了一种非动物替代小鼠生物测定参考方法的方法，对麻痹性贝类中毒毒素（PST）进行了常规监测； AOAC 2005.06柱前氧化液相色谱方法的改进版本。这种仪器方法的应用不仅可以生成有关PST事件的发生和程度的数据，而且还可以量化和评估不同的PST配置文件。自2008年实施该方法以来，结果表明PST的发生具有很大的变异性，海岸线周围的时空变异性很大。没有发现平均PST分布图与贝类的总PST含量，采样年份或贝类物种相关（除少数值得注意的情况外）。发现毒素分布图属于四种不同的分布图类型之一，其中一种仅与冲浪蛤中排他氨基甲酰基毒素的唯一存在有关。其他类型的类型包含不同比例的淋菌毒素，N-磺基氨基甲酰基毒素，新萨克毒素和沙毒素。虽然注意到了地理可重复性的一些迹象，但并非所有配置文件类型都观察到了这一点。因此，鉴于PST同系物交叉反应的巨大差异，需要仔细考虑将快速免疫化学测试方法应用于最终产品测试的情况。

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  • 来源
    《Harmful Algae》 |2014年第1期|87-99|共13页
  • 作者

    Andrew D. Turner; Ben Stubbs; Lewis Coates; Monika Dhanji-Rapkova; Robert G. Hatfield; Adam M. Lewis; Stephanie Rowland-Pilgrim; Alison ONeil; Patrycja Stubbs; Stuart Ross; Clothilde Baker; Myriam Algoet;

  • 作者单位

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

    Centre for Environment, Fisheries and Aquaculture Science (Cefas), Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, United Kingdom;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Liquid chromatography; Official controls; Paralytic shellfish poisoning; Toxin profiles;

    机译：液相色谱;官方控制;麻痹性贝类中毒;毒素概况;

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