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首页> 外文期刊>Harmful Algae >Morphology and molecular phylogeny of Nitzschia bizertensis sp. nov.-A new domoic acid-producer
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Morphology and molecular phylogeny of Nitzschia bizertensis sp. nov.-A new domoic acid-producer

机译：Nitzschia bizertensis sp。的形态和分子系统发育。十一月-一种新的海藻酸生产商

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摘要

A new toxin-producing marine diatom, Nitzschia bizertensis sp. nov., isolated from the Bizerte Lagoon (Tunisia, Southwest Mediterranean Sea) is, based on studies on eight different strains, characterized morphologically by light microscopy, transmission and scanning electron microscopy, and phylogeneti-cally using the nuclear rDNA regions: SSU, ITS1,5.8S, ITS2 and D1-D3 of the LSU. The species belongs to the sections Lanceolatae or Lineares as defined by Cleve and Grunow (1880). These sections are characterized by species having linear-lanceolate valves with an eccentric raphe where the fibulae does not extend into the valve, and are otherwise famous for the lack of characters useful for delineation of species. Nitzschia bizertensis differs from most other species in these sections by having a high density of interstriae. The morphological and phylogenetic studies and comparisons with previously described Nitzschia species showed Nitzschia bizertensis sp. nov. to be a new species. Batch culture experiments were conducted for estimations of maximum growth rate and production of domoic acid (DA). Maximum cellular DA content ofthe examined strains ranged from 2 × 10~(-4) to 3.6 × 10~(-2)pg cells~(-1). The total DA concentration (pg mL~(-1)) was high already in exponential growth phase maybe due to reinoculation of "old" stationary phase cells, and increased into stationary growth phase where it reached a stationary level varying among the strains from ca. 4500 to 9500 pg mL~(-1). Nitzschia bizertensis represents a new domoic acid-producing diatom and is the second toxin producing Nitzschia species. The resolution of Nitzschia bizertensis and Nitzschia navis-varingica in different parts of the LSU phylogenetic tree, and the recovery of the Pseudo-nitzschia species phylogenetically distant from those two species suggests that the ability to produce DA either evolved multiple times independently or was lost multiple times.
机译：一种新的产生毒素的海洋硅藻，Nitzschia bizertensis sp。基于对八种不同菌株的研究，从Bizerte泻湖（突尼斯，西南地中海）分离而来的Nov.，通过光学显微镜，透射和扫描电子显微镜进行形态学表征，并使用核rDNA区域进行系统发育：SSU，ITS1 LSU的5.8S，ITS2和D1-D3。该物种属于由Cleve和Grunow（1880）定义的Lanceolatae或Lineares部分。这些部分的特征是具有线性披针形瓣膜的物种，其中偏心圆角处的腓骨不延伸到瓣膜中，否则，这些物种因缺乏可用于描绘物种的特征而闻名。在这些区域中，bizitzensis不同于其他大多数物种，因为其间孔密度很高。形态学和系统发育研究以及与先前描述的尼兹菌属物种的比较显示了尼兹菌属bizertensis sp。十一月成为一个新物种。进行了分批培养实验，以估算最大生长速率和多摩酸（DA）的产量。被测菌株的最大细胞DA含量范围为2×10〜（-4）至3.6×10〜（-2）pg细胞〜（-1）。总DA浓度（pg mL〜（-1））在指数生长期已经很高，可能是由于“旧”固定相细胞的重新接种所致，并增加到了固定生长期，在此阶段它达到了一个稳定水平，在不同菌株之间。 4500至9500 pg mL〜（-1）。 Biztensis代表了一种新的产二氢产硅藻的硅藻，是第二个产生Nitzschia毒素的物种。 LSU系统发育树的不同部分中的拟南芥和Nitzschia navis-varingica的分辨率以及与这两个物种在系统上距离较远的假拟南芥物种的恢复表明，产生DA的能力要么独立发展多次，要么就丧失了多个次。

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  • 来源
    《Harmful Algae》 |2014年第2期|49-63|共15页
  • 作者

    Donia Bouchouicha Smida; Nina Lundholm; Wiebe H.C.F. Kooistra; Ines Sahraoui; Maria Valeria Ruggiero; Yuichi Kotaki; Marianne Ellegaard; Christophe Lambert; Hassine Hadj Mabrouk; Asma Sakka Hlaili;

  • 作者单位

    Laboratoire de Cytologie Vegetale et Phytoplanctonologie, Departement des Sciences de la Vie, Faculte des Sciences de Bizerte, Universite de Carthage,7021 Zarzouna, Bizerte, Tunisia;

    Natural History Museum of Denmark, University of Copenhagen, Solvgade 83S, DK-1307 Copenhagen K, Denmark;

    Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy;

    Laboratoire de Cytologie Vegetale et Phytoplanctonologie, Departement des Sciences de la Vie, Faculte des Sciences de Bizerte, Universite de Carthage,7021 Zarzouna, Bizerte, Tunisia;

    Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Napoli, Italy;

    School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan;

    Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark;

    Laboratoire des Sciences de l'Environnement Marin - UMR 6539, Institut Universitaire Europeen de la Mer, Technopole Brest-Iroise, 29280 Plouzane, France;

    Laboratoire de Cytologie Vegetale et Phytoplanctonologie, Departement des Sciences de la Vie, Faculte des Sciences de Bizerte, Universite de Carthage,7021 Zarzouna, Bizerte, Tunisia;

    Laboratoire de Cytologie Vegetale et Phytoplanctonologie, Departement des Sciences de la Vie, Faculte des Sciences de Bizerte, Universite de Carthage,7021 Zarzouna, Bizerte, Tunisia;

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  • 正文语种 eng
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  • 关键词

    Nitzschia bizertensis sp. nov.; Domoic acid; Toxin; Diatom; LSU rDNA; SSU rDNA; ITS rDNA; Phylogeny;

    机译：尼兹菌bizertensis sp。十一月海藻酸;毒素;硅藻;LSU rDNA;SSU rDNA;ITS rDNA;系统发育;

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