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首页> 外文期刊>Harmful Algae >Characterization of an algicidal bacterium Brevundimonas J4 and chemical defense of Synechococcus sp. BN60 against bacterium J4
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Characterization of an algicidal bacterium Brevundimonas J4 and chemical defense of Synechococcus sp. BN60 against bacterium J4

机译：杀菌细菌Brevundimonas J4的表征和Synchococcus sp。的化学防御。抗细菌J4的BN60

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摘要

As part of efforts to enhance the strategies explored to eliminate the adverse impacts of cyanobacterial blooms, we isolated an algicidal bacterium, J4, from Lake Taihu. Analysis of 16S rDNA sequence revealed that strain J4 belonged to the genus Brevundimonas. Bacterium J4 exhibited algicidal activity mainly through excretion of extracellular algicidal compounds that were further extracted with methanol and purified by silica gel chromatography and high performance liquid chromatography (HPLC). The compounds showed thermal stability, strong polarity and water solubility in J4 cultures. Study on the algicidal activity of J4 against two dominant cyanobacterial bloom-forming species in Lake Taihu showed that J4 exhibited lower algicidal rate against Synechococcus sp. BN60 (48.6%, t = 6 days) than against Microcystis aeruginosa 9110 (91.8%, r = 6 days). Additionally, rapid reduction in cell density of J4 was observed in co-cultures of Synechococcus sp. BN60 and bacterium J4 but not observed in co-cultures of M. aeruginosa 9110 and bacterium J4 during algicidal process, which was the main reason why the algicidal rate of J4 against BN60 was lower than against 9110. The reduction in cell density of J4 resulted from inducible production of antimicrobial-like compound secreted by Synechococcus sp. BN60 in co-cultures of Synechococcus sp. BN60 and bacterium J4, which reflected a kind of chemical defense from cyanobacteria (BN60) against algicidal bacteria (J4). However, M. aeruginosa 9110 had no chemical defense against J4, suggesting that whether cyanobacterial chemical defense occurs or not between cyanobacteria and algicidal bacteria depends on specific cyanobacteria-algicidal bacteria pairs. These results show that not only one-sided algicidal effect but also two-sided reciprocal inhibition interactions exist between algicidal bacteria and cyanobacteria, indicating the complexity of cyanobacteria-algicidal bacteria interactions in Lake Taihu and the need to take the cyanobacterial defensive responses into consideration when assessing potential use of algicidal bacteria.
机译：为了增强旨在消除蓝藻水华不利影响的策略，我们从太湖分离出了一种J4杀藻细菌。对16S rDNA序列的分析表明，菌株J4属于短杆菌属（Brevundimonas）。细菌J4主要通过排泄细胞外杀藻化合物表现出杀藻活性，这些化合物进一步用甲醇萃取并通过硅胶色谱和高效液相色谱（HPLC）进行纯化。这些化合物在J4培养物中显示出热稳定性，强极性和水溶性。对太湖J4对两种优势蓝藻水华形成物种的杀藻活性研究表明，J4对Synechococcus sp的杀藻率较低。 BN60（48.6％，t = 6天）比对铜绿微囊藻9110（91.8％，r = 6天）高。另外，在Synchococcus sp。的共培养物中观察到J4的细胞密度快速降低。 BN60和细菌J4在铜绿假单胞菌9110和细菌J4的共培养过程中未观察到，这是J4对BN60的杀藻率低于对9110的主要原因。J4细胞密度降低导致从诱导产生的Synchococcus sp分泌的抗菌剂样化合物。 Synechococcus sp。的共培养物中的BN60。 BN60和细菌J4，反映了蓝藻（BN60）对藻类细菌（J4）的一种化学防御作用。但是，铜绿假单胞菌9110对J4没有化学防御作用，这表明在蓝细菌和藻类细菌之间是否发生蓝细菌化学防御取决于特定的蓝细菌-藻类细菌对。这些结果表明，在藻类细菌和蓝细菌之间不仅存在单向的杀藻作用，而且存在双向的相互抑制作用，这表明太湖蓝藻-杀藻细菌相互作用的复杂性，并且在考虑时需要考虑蓝细菌的防御反应。评估杀藻细菌的潜在用途。

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  • 来源
    《Harmful Algae》 |2014年第7期|1-7|共7页
  • 作者

    Shengqin Lin; Jianliang Pan; Zhenghua Li; Xianglong Liu; Jing Tan; Hong Yang;

  • 作者单位

    State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Shanghai 200240, PR China;

    State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Shanghai 200240, PR China;

    State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Shanghai 200240, PR China;

    State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Shanghai 200240, PR China;

    State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Shanghai 200240, PR China;

    State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd. Shanghai 200240, PR China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Microcystis aeruginosa; Algicidal bacterium Brevundimonas; Algicidal compound; Synechococcus sp.; Chemical defense;

    机译：铜绿微囊藻;杀藻细菌短杆菌属;杀藻化合物;Synechococcus sp .;化学防御;

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