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首页> 外文期刊>Harmful Algae >Accumulation and detoxification dynamic of cyanotoxins in the freshwater shrimp Palaemonetes argentinus
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Accumulation and detoxification dynamic of cyanotoxins in the freshwater shrimp Palaemonetes argentinus

机译：淡水虾阿根廷银emo中毒素的积累和解毒动态

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摘要

The uptake and accumulation of microcystin-LR (MC-LR) in the shrimp Palaemonetes argentinus was investigated using both laboratory and field assays. Shrimps were exposed in aquarium during 1,2,3 and 7 days to 1,10 and 50 μg L~(-1) MCLR. Accumulation (0.7 ± 0.2 μg MC-LR g~(-1)) was observed after three days exposures to 50 μg L~(-1) toxin. Then, shrimps were relocated in fresh water (free of MCLR) to verify the detoxification dynamic, showing a drop to 0.18 ±0.01 μg MCLR g~(-1) after three days. The activity of glutathione-S-transferase, measured in the microsomal fraction (mGST), was significantly increased during the exposure period, with further increment during the detoxification period. Furthermore, cytosolic GST (sGST) and glutathione reductase (GR) increased their activities during detoxification, while inhibition was observed for catalase (CAT) with no significant changes for glutathione peroxidase (GPx). Current results suggest that GSH conjugation could be an important MC detoxification mechanism in P. argentinus and that MCLR induce oxidative stress in this shrimp. Field exposures were carried out in San Roque Reservoir (Cordoba, Argentina) after a cyanobacteria bloom. Nodularin (Nod) presence was measured for the first time in this waterbody (0.24 ± 0.04 μg L~(-1)), being the first report of Nod in South America freshwaters. Nod was also detected in Palaemonetes argentinus (0.16 ± 0.03 μg g~(-1)) after three weeks of exposure in this reservoir, with the concomitant activation of mGST, sGST and CAT. Although internal doses of Nod were low throughout the exposure, they were enough to cause biochemical disturbances in Palaemonetes argentinus. Further laboratory studies on Nod accumulation and antioxidant responses in Palaemonetes argentinus are necessary to fully understand these field results. P. argentinus should be considered a potential vector for transferring cyanotoxins to higher trophic levels in aquatic environments.
机译：使用实验室和现场试验研究了虾对虾Palaemonetes的微囊藻毒素-LR（MC-LR）的吸收和积累。虾在1,2,3和7天的时间内分别暴露于1,10和50μgL〜（-1）MCLR的水族馆中。暴露于50μgL〜（-1）毒素三天后观察到累积（0.7±0.2μgMC-LR g〜（-1））。然后，将虾移至淡水（不含MCLR）中以验证解毒动态，三天后虾的MCLR g〜（-1）下降至0.18±0.01μg。谷胱甘肽-S-转移酶的活性，在微粒体部分（mGST）中测量，在暴露期间显着增加，在排毒期间进一步增加。此外，在解毒过程中，胞质GST（sGST）和谷胱甘肽还原酶（GR）增强了它们的活性，而过氧化氢酶（CAT）受到抑制，而谷胱甘肽过氧化物酶（GPx）没有明显变化。目前的结果表明，GSH结合可能是阿根廷对虾中MC的重要解毒机制，而MCLR可以诱导这种虾的氧化应激。蓝细菌繁殖后，在圣罗克水库（阿根廷科尔多瓦）进行了野外接触。在该水体中首次测量了Nodularin（Nod）的存在（0.24±0.04μgL〜（-1）），这是南美淡水中Nod的首次报道。在该水库中暴露三周后，在阿根廷古猿（0.16±0.03μgg〜（-1））中也检测到Nod，同时激活了mGST，sGST和CAT。尽管Nod的内部剂量在整个暴露过程中都很低，但足以引起阿根廷Palaemonetes的生化紊乱。为了充分了解这些田间结果，有必要对阿根廷古猿网中的Nod积累和抗氧化反应进行进一步的实验室研究。阿根廷假单胞菌应被视为在水生环境中将氰毒素转移到较高营养水平的潜在载体。

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  • 来源
    《Harmful Algae》 |2013年第7期|88-97|共10页
  • 作者

    Lucas Nicolas Galanti; Maria Valeria Ame; Daniel Alberto Wunderlin;

  • 作者单位

    Universidad National de Cordoba - CONICET, Facultad de Ciencias Quimicas, Dpto. Bioquimica Clinica - QBIO, Haya de la Torre esq, Medina Allende, 5000 Cordoba, Argentina;

    Universidad National de Cordoba - CONICET, Facultad de Ciencias Quimicas, Dpto. Bioquimica Clinica - QBIO, Haya de la Torre esq, Medina Allende, 5000 Cordoba, Argentina;

    Universidad National de Cordoba - CONICET, Facultad de Ciencias Quimicas, Dpto. Quimica Orgdnica - ICYTAC, Bv. Dr. Juan Filloy s, Ciudad Universitaria, 5000 Cordoba, Argentina;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bioaccumulation; Cyanotoxins; Enzymatic activity; Invertebrates; LC-MS/MS;

    机译：生物蓄积;氰毒素;酶活性;无脊椎动物;液质联用;

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