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Occurrence of toxic Prymnesium parvum blooms with high protease activity is related to fish mortality in Hungarian ponds

机译:具有高蛋白酶活性的有毒小白花绽放的发生与匈牙利池塘中的鱼类死亡率有关

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摘要

The unicellular alga Prymnesium parvum has been responsible for toxic incidents with severe ecological impacts in many parts of the world, and causes massive fish kills worldwide. Recently the haptophyte microalgae have caused water-bloom (4.3 × 10~(-1) cells ml~(-1)) in 6 fish ponds with high conductivity in Hungary, and caused fish mortality with typical symptoms. Toxicity of P. parvum from water samples was quantified by the assay of the influence of its cell-free filtrates on haemolysis (346 ±42.2) and in fish and daphnia toxicity tests. High amount of proteases in P. parvum containing waterbloom samples were detected with the help of activity gel electrophoresis. The proteases of investigated P. parvum samples (125-18 kDa) showed high gelatinolytic activity and some of them showed sensitivity to EDTA (inhibitors of metalloproteases) and to PMSF (inhibitors of serine proteases).
机译:在世界许多地方,单细胞藻小球藻造成了严重的生态影响的有毒事件,并在世界范围内造成大量鱼类死亡。最近,触藻植物微藻在匈牙利的6个高电导率鱼塘中引起水华(4.3×10〜(-1)细胞ml〜(-1)),并导致鱼类死亡,并具有典型症状。水样小菜蛾的毒性通过测定其无细胞滤液对溶血的影响(346±42.2)以及在鱼类和水蚤的毒性试验中进行定量。借助活性凝胶电泳检测到含有小球藻的水华样品中的大量蛋白酶。研究的小球藻样品的蛋白酶(125-18 kDa)显示出高的明胶分解活性,其中一些蛋白酶对EDTA(金属蛋白酶抑制剂)和PMSF(丝氨酸蛋白酶抑制剂)敏感。

著录项

  • 来源
    《Harmful Algae》 |2012年第5期|102-110|共9页
  • 作者单位

    University of Debrecen, Department of Botany, Faculty of Science and Technology, PO Box 14, H-4010 Debrecen, Hungary,Debrecen University, Department of Botany, Egyetem sq 1. Debrecen 4010, Hungary;

    University of Debrecen, Department of Botany, Faculty of Science and Technology, PO Box 14, H-4010 Debrecen, Hungary;

    Hungarian Academy of Sciences, Balaton Limnological Research Institute, Department of Tisza River Research, 18/c. Bern square, Debrecen H-4026, Hungary;

    University of Debrecen, Department of Botany, Faculty of Science and Technology, PO Box 14, H-4010 Debrecen, Hungary;

    University of Debrecen, Department of Botany, Faculty of Science and Technology, PO Box 14, H-4010 Debrecen, Hungary;

    University of Debrecen, Department of Botany, Faculty of Science and Technology, PO Box 14, H-4010 Debrecen, Hungary;

    Environmental Protection, Nature Conservation and Water Authority, Lower Tisza Region, Pf, 1048, H-6712 Szeged, Hungary;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fishkill; protease; prymnesium parvum; zymographies;

    机译:杀鱼蛋白酶小花ry酶谱学;

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