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首页> 外文期刊>Harmful Algae >Temporal variability of cyanobacterial populations in the water and sediment samples of Lake Taihu as determined by DGGE and real-time PCR
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Temporal variability of cyanobacterial populations in the water and sediment samples of Lake Taihu as determined by DGGE and real-time PCR

机译：DGGE和实时荧光定量PCR测定太湖水和沉积物样品中蓝细菌种群的时间变化

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摘要

This study was conducted to provide a detailed understanding of the variation in cyanobacterial communities in the water and sediment of Lake Taihu. DGGE and real-time PCR techniques were conducted to describe the distributions of the cyanobacterial community in the water and their relationship with the development of blooms over one year. In addition, the vertical distributions of cyanobacterial communities in the sediment were determined. The results suggested that the diversity of the cyanobacterial population in the pelagic water was quite low from July to September, and that Microcystis and Synechococcus were the two most dominant cyanobacteria. The copy number of Synechococcus was approximately one order of magnitude higher than that of Microcystis during the blooming seasons (June-September), whereas, with the exception of December, the copy number of Microcystis was slightly higher than that of Synechococcus during the non-bloom seasons. The highest cyanobacterial diversity was observed in the sediment at 20-25 cm. Furthermore, the amount of Synechococcus was higher than that of Microcystis in the upper layers of the sediment (0-20 cm), whereas the amount of Microcystis was higher than that of Synechococcus in samples collected from the deeper layers (20-30 cm).
机译：进行这项研究是为了提供对太湖水和沉积物中蓝细菌群落变化的详细了解。进行了DGGE和实时PCR技术，以描述水中蓝藻群落的分布及其与一年以上水华发生的关系。此外，确定了沉积物中蓝细菌群落的垂直分布。结果表明，7月至9月，中上层水中蓝细菌种群的多样性很低，微囊藻和聚球菌是两个最主要的蓝细菌。在开花季节（6月至9月），集胞球菌的拷贝数比微囊藻的拷贝数高大约一个数量级，而除12月外，在非开花期微囊藻的拷贝数比集胞菌的拷贝数略高。盛开的季节。在20-25 cm的沉积物中观察到最高的蓝细菌多样性。此外，在沉积物上层（0-20厘米）中，球囊菌的数量高于微囊藻，而在较深层（20-30厘米）中采集的样品中，微囊藻的数量高于球囊菌。 。

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  • 来源
    《Harmful Algae》 |2011年第5期|p.472-479|共8页
  • 作者

    Wenjin Ye; Jing Tan; Xianglong Liu; Shengqin Lin; Jianliang Pan; Daotang Li; Hong Yang;

  • 作者单位

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

    MOE Key Laboratory ofMicrobial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., Shanghai 200240, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cyanobacteria; lake taihu; water and sediment; DGCE; real-time pcr;

    机译：蓝细菌太湖水和沉积物;DGCE;实时pcr;

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