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首页> 外文期刊>Harmful Algae >Detection of Cochlodinium polykrikoides and Gymnodinium impudicum (Dinophyceae) in sediment samples from Korea using real-time PCR
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Detection of Cochlodinium polykrikoides and Gymnodinium impudicum (Dinophyceae) in sediment samples from Korea using real-time PCR

机译：实时荧光定量PCR检测韩国沉积物样品中的Cochlodinium polykrikoides和Gymnodinium impudicum（Dinophyceae）

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摘要

Recurring blooms of fish killing dinoflagellate Cochlodinium polykrikoides has resulted in large economic losses in fisheries industry in Korea. This species has been monitored in water column samples, but its spatial distribution in sediments is poorly understood. To address this area, geographic distribution of C. polykrikoides and morphologically similar species Gymnodinium impudicum in surface sediments of Korea was investigated using species-specific real-time PCR probes targeting the internal transcribed spacer 2 rRNA gene. PCR-inhibitory substances in sediment samples were removed by dilution of DNA extracts from the field samples for preventing false-negative detection. G. impudicum was widely distributed in sediments from East, South, and Yellow Seas. C. polykrikoides was prevalent in sediments from South Sea whereas it was not detected in sediments from East and Yellow Seas. These results indicate that these dinoflagellates may persist in surface sediment likely in the form of cyst and their "seed beds" may exist in sediments of South Sea where blooms of C. polykrikoides occur annually.
机译：鱼类的反复开花杀死了鞭毛甲藻鞭藻，导致了韩国渔业的巨大经济损失。该物种已在水柱样品中进行了监测，但对其在沉积物中的空间分布知之甚少。为了解决这一问题，利用靶向内部转录间隔子2 rRNA基因的物种特异性实时PCR探针，对韩国表面沉积物中的多角衣藻和形态相似的物种裸子草的地理分布进行了研究。通过稀释野外样品中的DNA提取物来去除沉积物样品中的PCR抑制性物质，以防止假阴性检测。不定根G. impudicum广泛分布在东，南和黄海的沉积物中。 C. polykrikoides在南海的沉积物中普遍存在，而在东海和黄海的沉积物中则未检出。这些结果表明，这些鞭毛藻可能以囊肿的形式存在于地表沉积物中，并且它们的“种子床”可能存在于南海的沉积物中，那里每年都发生多角衣藻的开花。

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