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首页> 外文期刊>Harmful Algae >Detection of Pseudo-nitzschia (Bacillariophyceae) species and amnesic shellfish toxins in Scottish coastal waters using oligonucleotide probes and the Jellet Rapid Test~(TM)
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Detection of Pseudo-nitzschia (Bacillariophyceae) species and amnesic shellfish toxins in Scottish coastal waters using oligonucleotide probes and the Jellet Rapid Test~(TM)

机译：使用寡核苷酸探针和Jellet Rapid Test〜（TM）检测苏格兰沿海水域的假奈瑟菌（Bacillariophyceae）物种和遗忘性贝类毒素

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摘要

Species specific LSU rRNA targeted fluorescent oligonucleotide probes, designed by researchers at the Monterey Bay Aquarium Research Institute (USA) for a limited range of Pseudo-nitzschia species, were applied to unialgal cultures and Scottish field samples, to investigate possible applications in Scottish phytoplankton monitoring programmes to detect potential amnesic shellfish poisoning (ASP) toxin producing species. The existing available probe for Pseudo-nitzschia australis gave good results, positively labelling cells from cultures and field samples. However, application of the P. pungens, P. delicatissima and P. fraudulenta probes gave poor results, with little or no fluorescence label observed in field samples, while transmission electron microscopy (TEM) showed these species to be present. Comparison of the same region of the LSU sequence from cultures of P. delicatissima, isolated from Scottish waters, with the probe designed for detection of P. delicatissima isolated from Monterey Bay revealed the presence of a single base difference between the two sequences, which may have prevented the probe from hybridising to Scottish isolates and cells from field samples. In an attempt to assess the potential ASP toxin production by field populations of Pseudo-nitzschia a rapid immunodiagnostic test (the Jellet Rapid Test, JRT) for ASP toxins was examined. Results indicate that additional development of molecular probes for the detection of a range of Pseudo-nitzschia species detected in Scottish coastal waters and the use of JRT for toxin detection could conceivably provide an effective tool for broad-scale mapping of toxin events and management of coastal zone activities.
机译：由蒙特利湾水族馆研究所（美国）的研究人员针对有限范围的拟南芥属物种设计的针对物种的特定LSU rRNA荧光寡核苷酸探针被用于非藻类培养物和苏格兰田间样品，以研究在苏格兰浮游植物监测中的可能应用程序来检测潜在的遗忘性贝类中毒（ASP）毒素产生物种。现有的用于南方假单胞菌的探针给出了良好的结果，对来自培养物和田间样品的细胞进行了阳性标记。然而，应用P. pungens，P。delicatissima和P.欺诈ulenta探针的结果较差，在野外样品中观察到很少或没有荧光标记，而透射电镜（TEM）显示存在这些物质。从苏格兰水域分离出的P. delicatissima培养物中LSU序列的相同区域与设计用于检测从蒙特雷湾分离的P. delicatissima的探针的比较显示，这两个序列之间存在一个碱基差异，这可能已阻止探针与来自现场样品的苏格兰分离物和细胞杂交。为了评估假性奈瑟菌田间种群可能产生的ASP毒素，我们对ASP毒素进行了快速免疫诊断测试（Jellet快速测试，JRT）。结果表明，可以进一步开发分子探针来检测在苏格兰沿海水域中检测到的一系列假性奈瑟菌物种，并使用JRT进行毒素检测，可以为大规模毒素事件作图和沿海地区管理提供有效的工具。区域活动。

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