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Optical characteristics of the harmful dinoflagellate Alexandrium tamarense in response to different nitrogen sources

机译:有害的鞭毛藻亚历山大藻对不同氮源的响应

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摘要

The chlorophyll a specific absorption coefficient a_(ph)~* and absorption ratio (a_(ph) ratio) of Alexandrium tamarense at five concentrations of nitrate, ammonium and urea over a range from 6 to 100 μM were examined. The experimental results were compared to two coastal diatom species and a prymnesiophyte to identify differences in absorption ratios. Cells exposed to increasing nitrate concentrations were characterized by an increase in a_(ph)~* at 443, 490, 510, 555 and 675 nm. However, ammonium and urea induced low a_(ph)~* values at their lowest and highest concentrations. The a_(ph) relative to 510 or 555 nm was constant regardless of the concentration of the N source, but dependent on the N source. Oxidized N induced a lower a_(ph) ratio than the reduced form. Comparisons of the a_(ph) ratio among taxonomic groups revealed significant differences. The a_(ph) ratio of A. tamarense was 20-30 and > 50% lower than those of two diatoms and a prymnesiophyte, respectively. The a_(ph) ratio of the present study could assist in increasing the capability for detecting harmful species such as A. tamarense.
机译:检测了硝酸盐,铵和尿素在5至100μM范围内的五个浓度下的亚历山大藻的叶绿素a比吸收系数a_(ph)〜*和吸收比(a_(ph)比)。将实验结果与两个沿海硅藻物种和一个褐藻类植物进行比较,以确定吸收率的差异。暴露于增加的硝酸盐浓度下的细胞的特征在于在443、490、510、555和675 nm处α_(ph)〜*的增加。然而,铵和尿素在最低和最高浓度下诱导出较低的a_(ph)〜*值。不管N源的浓度如何,相对于510或555nm的a_(ph)是恒定的,但是取决于N源。氧化氮诱导的α_(ph)比率低于还原态。分类组之间的a_(ph)比的比较显示出显着差异。 tamarense的a_ph比率分别比两个硅藻和prymnesiophyte的a_ph比率低20-30,且> 50%。本研究的a_(ph)比可以帮助提高检测有害物种(如番茄)的能力。

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