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首页> 外文期刊>Harmful Algae >Growth and toxin production in batch cultures of a marine dinoflagellate Alexandrium tamarense HK9301 isolated from the South China Sea
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Growth and toxin production in batch cultures of a marine dinoflagellate Alexandrium tamarense HK9301 isolated from the South China Sea

机译：从南中国海分离的海洋鞭毛藻tamarense HK9301的分批培养中的生长和毒素产生

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Nutritional and environmental conditions were characterized for a batch culture of the marine dinoflagellate Alexandrium tamarense HK9301 isolated from the South China Sea for its growth (cells ml~(-1)), cellular toxin content (Qt in fmol cell~(-1)) and toxin composition (mol%). Under a nutrient replete condition, Qt increased with cell growth and peaked at the late stationary phase. Toxin content increased with the nitrate concentration in the culture while it reached a maximum at 5 μM phosphate. When nitrate was replaced with ammonia, Qt decreased by 4.5-fold. Salinity and light intensity were important factors affecting Qt. The latter increased two-fold over the range of salinity from 15 to 30 per thousand, while decreased 38% as light intensity increased from 80 to 220 μE m~(-2) s~(-1). Toxin composition varied with growth phase and culture conditions. In nutrient replete cultures, toxin composition varied greatly in the early growth phase (first 3 days) and then C1/C2, C3/C4 and GTX1 remained relatively constant while GTX4 increased from 32 to 46% and GTX5 decreased from 28 to 15%. In general, the composition of GTXs was affected in a much greater extent than C toxins by changes in nutrient conditions, salinity and light intensity. This is especially true with GTX4 and GTX5. These data indicate that the cellular toxin content and toxin composition of A. tamarense HK9301 are not constant, but that they vary with growth phase and culture conditions. Use of toxin composition to identify a toxigenic marine dinoflagellate is not always valid. The data also reveal that high salinity and low light intensity, together with high nitrate and low phosphate concentrations, would favor toxin production by this species.
机译：通过分批培养从南海分离出的海洋鞭毛藻tamarense HK9301的营养和环境条件进行表征，该生长物的生长（细胞ml〜（-1）），细胞毒素含量（fmol细胞〜（-1）中的Qt）和毒素组成（摩尔％）。在营养充足的条件下，Qt随着细胞生长而增加，并在静止后期达到峰值。毒素含量随培养物中硝酸盐浓度的增加而增加，而在5μM磷酸盐时达到最大值。当用氨水代替硝酸盐时，Qt降低了4.5倍。盐度和光强度是影响Qt的重要因素。后者在盐度从千分之15到30的范围内增加了两倍，而随着光强度从80μEm〜（-2）s〜（-1）增加而降低了38％。毒素组成随生长期和培养条件而变化。在营养丰富的培养物中，毒素组成在早期生长阶段（前3天）变化很大，然后C1 / C2，C3 / C4和GTX1保持相对恒定，而GTX4从32％增加到46％，GTX5从28％减少到15％。通常，营养条件，盐度和光照强度的变化对GTX的组成的影响远大于C毒素。对于GTX4和GTX5尤其如此。这些数据表明，A。tamarense HK9301的细胞毒素含量和毒素组成不是恒定的，但是它们随生长期和培养条件而变化。使用毒素组合物鉴定产毒性海洋鞭毛鞭毛藻并非总是有效的。数据还显示高盐度和低光强度，以及高硝酸盐和低磷酸盐浓度，将有利于该物种产生毒素。

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