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首页> 外文期刊>Harmful Algae >Utilization of various forms of nitrogen and expression regulation of transporters in the harmful alga Heterosigma akashiwo (Raphidophyceae)
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Utilization of various forms of nitrogen and expression regulation of transporters in the harmful alga Heterosigma akashiwo (Raphidophyceae)

机译：有害藻类赤潮菜中各种氮素的利用及转运蛋白的表达调控

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摘要

Nitrogen (N) is an essential nutrient for phytoplankton growth. There is ample evidence that N enrichment promotes harmful algae blooms (HABs) but molecular mechanisms regulating N-nutrient uptake and metabolism are not so clear, especially for the raphidophyte Heterosigma akashiwo, which forms ichthyotoxic HABs in many coastal waters. In this study, the utilization of three different chemical forms of N (nitrate, ammonium, and urea) by H. akashiwo CCMA 369 was investigated in batch culture conditions. Results showed that H. akashiwo grew well on all three N compounds, and the highest cell yield occurred in the NH4+ culture group. Reverse transcription quantitative PCR analysis revealed that the expression of high-affinity NO3- transporter (NIT), NH4+ transporter (AMT) and high-affinity urea active transporter (DUR3), were significantly up-regulated under N-limitation compared to the N-replete control. The mRNA levels of AMT and DUR3 also displayed a clear diel rhythm, with high levels at midnight. In addition, NH4+ addition (5 mu M) did not depress the transcript abundance of any of the three N transporters. Compared with the co-occurring immobile diatom Skeletonema costatum, the high expression of AMT in dark period in H. akashiwo is consistent with its diel vertical migration behavior, which may promote N-nutrient acquisition from deeper layers and give advantages for H. akashiwo to form blooms.
机译：氮（N）是浮游植物生长所必需的营养素。有充分的证据表明，富氮促进有害藻类繁殖（HABs），但调节氮素吸收和代谢的分子机制尚不十分清楚，特别是对于在许多沿海水域形成鱼腥毒性HAB的水生植物杂种赤足鱼。在这项研究中，研究了H. akashiwo CCMA 369在分批培养条件下对三种不同化学形式的氮（硝酸盐，铵和尿素）的利用。结果表明，在所有三种N化合物上，akashiwo菌均生长良好，并且在NH4 +培养组中细胞产量最高。逆转录定量PCR分析显示，与N-相比，N-限制下高亲和力NO3-转运蛋白（NIT），NH4 +转运蛋白（AMT）和高亲和尿素活性转运蛋白（DUR3）的表达明显上调。完全控制。 AMT和DUR3的mRNA水平也显示出清晰的diel节奏，在午夜时具有很高的水平。另外，添加NH 4 +（5μM）不降低任何三个N转运蛋白的转录本丰度。与同时发生的固定硅藻骨骼藻相比，暗箱中的AMT在暗期的高表达与其diel垂直迁移行为相吻合，这可能促进深层氮素的吸收，并有利于暗箱菌的生长。形式绽放。

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  • 来源
    《Harmful Algae》 |2020年第2期|101770.1-101770.7|共7页
  • 作者

    Ji Nanjing; Zhang Zhenzhen; Huang Jinwang; Zhou Lingjie; Deng Shengxian; Shen Xin; Lin Senjie;

  • 作者单位

    Jiangsu Ocean Univ Jiangsu Key Lab Marine Bioresources & Environm Jiangsu Key Lab Marine Biotechnol Lianyungang 222005 Peoples R China|Jiangsu Ocean Univ Coinnovat Ctr Jiangsu Marine Bioind Technol Lianyungang 222005 Peoples R China|Xiamen Univ State Key Lab Marine Environm Sci Xiamen 361005 Fujian Peoples R China|Univ Connecticut Dept Marine Sci Groton CT 06340 USA;

    Jiangsu Ocean Univ Jiangsu Key Lab Marine Bioresources & Environm Jiangsu Key Lab Marine Biotechnol Lianyungang 222005 Peoples R China|Jiangsu Ocean Univ Coinnovat Ctr Jiangsu Marine Bioind Technol Lianyungang 222005 Peoples R China;

    Univ Connecticut Dept Marine Sci Groton CT 06340 USA;

    Xiamen Univ State Key Lab Marine Environm Sci Xiamen 361005 Fujian Peoples R China|Univ Connecticut Dept Marine Sci Groton CT 06340 USA;

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  • 正文语种 eng
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  • 关键词

    Heterosigma akashiwo; Nitrogen; Ammonium transporter; Diel rhythm;

    机译：赤子葵氮;铵转运蛋白;迪尔节奏;

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