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Lectin-stimulated cellular iron uptake and toxin generation in the freshwater cyanobacterium Microcystis aeruginosa

机译：淡水蓝杆菌微囊杆菌铜绿假单胞菌凝集素刺激的细胞铸铁和毒素产生

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摘要

The lectin family is composed of mono- and oligosaccharide binding proteins that could activate specific cellular activities, such as cell-cell attachment and toxin production. In the present study, the effect of the external addition of lectins to culture media containing the freshwater cyanobacterium Microcystis aeruginosa on its metabolic activities, such as iron uptake and toxin production was investigated. Among the three lectins examined in this study (concanavalin A [Con A], wheat germ agglutinin [WGA] and peanut agglutinin [PNA]), PNA substantially increased the accumulated intracellular and extracellular iron content. The binding of PNA and Con A to M. aeruginosa cells was visualized via fluorescence microscopy using a lectin adjunct with fluorescein isothiocyanate, and resulted in carbohydrate and protein accumulation in the cellular capsule. Given that the highest carbohydrate accumulation was seen in the Con A system (where iron accumulation was relatively lower), carbohydrate quality is likely important factor that influences cellular iron accumulation. Since PNA specifically binds to sugars such as galactose and N-acetylgalactosamine, these saccharide species could be important candidates for intracellular and extracellular iron accumulation and transport Microcystin biosynthesis was stimulated in the presence of PNA and WGA, whereas cellular iron uptake increased only in the presence of PNA. Thus, the iron uptake was not necessarily congruent with the upregulation of microcystin synthesis, which suggested that the positive effect of lectin on iron uptake is probably attributable to the PNA-assisted iron accumulation around the cell surface. Overall, the present study provides insights into the interactions of lectin that influence cellular metabolic activities such as iron uptake, extracellular polymeric substance accumulation, and toxin production.
机译：凝集素家族由单糖和寡糖结合蛋白组成，可激活特异性细胞活性，例如细胞 - 细胞附着和毒素生产。在本研究中，研究了凝集素对含有淡水蓝杆菌微囊杆菌铜绿假单胞菌的培养基对其代谢活性的影响，例如铁摄取和毒素生产。在本研究中检查的三种凝集素中（Concanavalin A [Con A]，小麦胚芽凝集素[WGA]和花生凝集素[PNA]），PNA基本上增加了积聚的细胞内和细胞外铁含量。通过荧光显微镜通过荧光显微镜使用荧光显微镜使用荧光素硫氰酸酯的荧光显微镜观察PNA和CON A至M.铜绿假单胞菌的结合，并导致细胞胶囊中的碳水化合物和蛋白质积累。鉴于在CON系统中看到最高的碳水化合物积累（其中铁积累相对较低），碳水化合物质量可能是影响细胞铁积累的重要因素。由于PNA特异性与半乳糖和N-乙酰甘酰胺胺的糖结合，因此这些糖类可以是细胞内和细胞外铁积累的重要候选者，并且在PNA和WGA存在下刺激传输微胱素生物合成，而细胞铁摄取仅在存在下增加PNA。因此，铸铁不一定是随着微囊藻合成的上调的一定是一致的，这表明凝集素对铁摄取的积极作用可能是围绕细胞表面的PNA辅助铁积累的贡献。总的来说，本研究提供了影响凝集素的相互作用的见解，这些研究会影响抗氧化镁活性，例如铁吸收，细胞外聚合物物质积累和毒素生产。

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