• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Harmful Algae >Development of a multiplex polymerase chain reaction assay for the parallel detection of harmful algal bloom-forming species distributed along the Chinese coast
【24h】

Development of a multiplex polymerase chain reaction assay for the parallel detection of harmful algal bloom-forming species distributed along the Chinese coast

机译：开发多重聚合酶链式反应测定，用于平行检测沿着中国海岸分布的有害藻类盛开的物种

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Harmful algal blooms (HABs) have adverse effects on the marine ecological environment, public health, and marine economy. Thus, methods for the accurate and rapid identification of harmful algal species are urgently needed for the effective monitoring of the occurrence of HABS. A method for the parallel detection of harmful algal species must be established because various HAB-forming algal species coexist in the marine environment. This work developed a multiplex PCR (mPCR) method that can simultaneously detect six common HAB-forming microalgal species distributed along the coast of China: Karlodiniwn veneficum (Kv), Chattonella marina (Cm), Skeletonema spp., Scrippsiella trochoidea (St), Karenia mikimotoi (Km), and Prorocentrum donghaiense (Pd). Specific mPCR primers were designed from the internal transcribed spacer rDNA or large subunit rDNA gene of the target algal species. The mPCR conditions were optimized. Each mPCR primer was subjected to a cross-reactivity test with other microalgae to confirm the specificity of the developed mPCR system. The results of the system stability test indicated that the background concentration of DNA tested did not affect the performance of the established mPCR system. The results of the sensitivity test showed that the detection limit of the proposed mPCR system for Kv, Cm, Km, and Pd was 0.6 ng mu L-1 and that for Skeletonema spp. and St was 0.06 ng mu L-1. Additional mPCR analysis with spiked field samples revealed that the detection limit of the mPCR system for Km, Pd, and Kv was 60 cells, whereas that for Cm, Skeletonema spp., and St was 6 cells. The convenience and accuracy of the established mPCR assay were further validated through tests with field samples. The proposed mPCR assay is characterized by parallel analysis, strong specificity, and stability and can be used to supplement morphology-based detection methods for algal species.
机译：有害的藻类盛开（HAB）对海洋生态环境，公共卫生和海洋经济产生不利影响。因此，迫切需要对有效监测疾病的有效监测来准确和快速鉴定有害藻类物种的方法。必须建立一种平行检测有害藻类物种的方法，因为海洋环境中的各种HAB形成藻类物种共存。这项工作开发了一种多重PCR（MPCR）方法，可同时检测沿着中国海岸分布的六种常见的HAB形成微藻物种：Karlodiniwn Veneficum（KV），Chattonella Marina（CM），骨质瘤SPP。，Scrippsiella Trochoidea（ST）， Karenia Mikimotoi（km）和prorocentrum dongaient（PD）。特异性MPCR引物由靶藻类物种的内部转录间隔rDNA或大亚基RDNA基因设计。优化MPCR条件。将每个MPCR引物与其他微藻进行交叉反应性试验，以确认发育MPCR系统的特异性。系统稳定性测试的结果表明，DNA测试的背景浓度不影响建立的MPCR系统的性能。灵敏度试验的结果表明，用于KV，CM，KM和Pd的提出的MPCR系统的检测限为0.6ng mu L-1，用于骨质肿瘤SPP。 ST为0.06 ng mu l-1。具有尖锐场样品的额外MPCR分析显示，MPCR系统的检测限为KM，Pd和KV为60个细胞，而对于CM，骨质肿仓SPP，ST为6个细胞。通过野外样品的试验进一步验证了已建立的MPCR测定的便利性和准确性。所提出的MPCR测定的特征在于平行分析，强特异性和稳定性，并且可用于补充基于形态的藻类检测方法。

著录项

  • 来源
    《Harmful Algae》 |2019年第4期|36-45|共10页
  • 作者

    Sun Yan-Jie; Chen Guo-Fu; Zhang Chun-Yun; Guo Chang-Lu; Wang Yuan-Yuan; Sun Rui;

  • 作者单位

    Harbin Inst Technol Weihai Sch Marine Sci & Technol Wenhua West Rd 2 Weihai 264209 Shandong Peoples R China;

    Harbin Inst Technol Weihai Sch Marine Sci & Technol Wenhua West Rd 2 Weihai 264209 Shandong Peoples R China;

    Harbin Inst Technol Weihai Sch Marine Sci & Technol Wenhua West Rd 2 Weihai 264209 Shandong Peoples R China;

    Harbin Inst Technol Weihai Sch Marine Sci & Technol Wenhua West Rd 2 Weihai 264209 Shandong Peoples R China;

    Harbin Inst Technol Weihai Sch Marine Sci & Technol Wenhua West Rd 2 Weihai 264209 Shandong Peoples R China;

    Harbin Inst Technol Weihai Sch Marine Sci & Technol Wenhua West Rd 2 Weihai 264209 Shandong Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Harmful algal blooms; Harmful microalgae; Multiplex PCR; Detection;

    机译：有害的藻类盛开;有害的微藻;多重PCR;检测;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号