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首页> 外文期刊>Harmful Algae >Phosphorus strategy in bloom-forming cyanobacteria (Dolichospermum and Microcystis) and its role in their succession
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Phosphorus strategy in bloom-forming cyanobacteria (Dolichospermum and Microcystis) and its role in their succession

机译：盛开的蓝藻（Dolichospermum和微囊杆菌）中的磷策略及其在继承中的作用

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摘要

Dolichospermum (formerly Anabaena) and Microcystis cause harmful cyanobacterial blooms in freshwater ecosystems worldwide. Input reduction of both nitrogen (N) and phosphorus (P) are commonly recognized as basic ways of controlling blooms, but little is known about the roles of nutrients and their using strategy among cyanobacteria in triggering the succession of diazotrophic to non-diazotrophic cyanobacteria. In this study, we investigated in situ responses of cyanobactria to ambient P status during the transition from Dolichospermum flos-aquae to Microcystis spp. in Lake Taihu and Lake Chaohu. While dominant in phytoplankton community, D. fibsaquae experienced P deficiency as evidenced by qualitative detection of extracellular phosphatase via enzyme labeled fluorescence (ELF). The percentage of ELF-labelled D. fios-aquae cells was 33% when it dominated the phytoplankton community, and was 78% when it co-dominated with Microcystis spp., indicating an increase in P deficiency. Meanwhile, no ELF-labelled Microcystis cells were observed while polyphosphate body (PPB) were present, suggesting that Microcystis spp. were not P deficient. Additionally, the percentages of Microcystis cells containing PPB showed an inverted "U-shaped" relationship with concentrations on soluble reactive phosphorus (SRP). To validate the field observation, a laboratory study of the monocultures of the dominant cyanobacteria was conducted. Extracellular alkaline phosphatase activity (APA) and PPB accumulation were regulated by P availability in monocultures of D. flos-aquae. Interestingly, no cell bound extracellular phosphatase was found on Microcystis aeruginasa even in the culture without P supply. Consistently, the expressions of phosphatase encoding gene phoX showed no differences among the treatments. The way in which PPB accumulation occurred in Microcystis spp. in response to P availability in the cultures was similar to that observed in the field, demonstrating a strategy of energy conservation over P accumulation. The competitive advantage of Microcystis spp. was displayed at low P concentrations: where it could rapidly uptake and store inorganic P, which also increased the P deficiency of the coexisting phytoplankton species. Responses of P-transport gene pstS confirmed this hypothesis. The physiological and molecular mechanisms mentioned above enable Microcystis to survive and proliferate in environment with low available P supply more efficiently. In conclusion, different cyanobacterial species have distinct ways of responding to P availability, suggesting that the control of cyanobacterial blooms by targeted nutrient reduction is largely dependent upon the dominant species. P reduction is more effective in controlling diazotrophic cyanobacteria than non-diazotrophic cyanobacteria.
机译：Dolichospermum（以前的Anabaena）和微囊杆菌造成全球淡水生态系统中有害的蓝藻绽放。氮气（N）和磷（P）的输入减少通常被认为是控制盛开的基本方法，但是关于营养素的作用以及它们在抗植物中的促进营养素中的连续脱氮性与非重氮脱节性的策略中的作用很少。在这项研究中，我们在从Dolichospermum Flos-Aquae转换到微阴压SPP期间的蕈类对紫花形蓍核的原位反应。在太湖湖和巢湖湖。虽然在Phytoplancton群落中占主导地位，但D.Fibsaquae经历了P缺乏，通过标记荧光（ELF）的质子磷酸酶定性检测证明。当它占据浮游植物群落时，ELF标记的D.FiOS-Aquafe细胞的百分比为33％，并且当它与微囊杆菌SPP共同定位时为78％。，表明P缺乏增加。同时，在存在多磷酸盐体（PPB）的同时，没有观察到ELF标记的微囊囊细胞，表明微囊体SPP。不是p缺乏。另外，含有PPB的微囊囊细胞的百分比显示与可溶性反应性磷（SRP）的浓度倒置的“U形”关系。为了验证田间观察，进行了对显性蓝细菌的单一栽培的实验室研究。通过D.Flos-Aquae的单一培养物中的P获取来调节细胞外碱性磷酸酶活性（APA）和PPB积累。有趣的是，即使在没有P供应的培养物中，也没有在微囊型铜绿素中发现细胞结合的细胞外磷酸酶。一致地，编码基因Phox的磷酸酶的表达在治疗中没有差异。 Microcystis SPP中PPB累积发生的方式。响应于文化中的P可用性类似于在该领域观察到的相似，展示了通过P积累的节能策略。微囊杆菌SPP的竞争优势。在低P浓度下显示：它可以快速摄取和储存无机P，这也增加了共存浮游植物的P缺乏。对P转移基因PSTS的反应证实了这一假设。上述生理和分子机制使微囊体能够在低可用的P供给更有效的情况下在环境中存活和增殖。总之，不同的蓝细菌种具有响应P可用性的不同方式，表明通过靶向营养还原对蓝藻绽放的控制在很大程度上取决于优势物种。 P减少在控制虚拟脱氮性蓝藻中比非重氮脱节性胞细胞更有效。

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  • 来源
    《Harmful Algae》 |2019年第4期|46-55|共10页
  • 作者

    Wan Lingling; Chen Xiaoyan; Deng Qinghui; Yang Liu; Li Xiaowen; Zhang Junyi; Song Chunlei; Zhou Yiyong; Cao Xiuyun;

  • 作者单位

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China|Univ Chinese Acad Sci Beijing 100039 Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China|Univ Chinese Acad Sci Beijing 100039 Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China|Univ Chinese Acad Sci Beijing 100039 Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China|Univ Chinese Acad Sci Beijing 100039 Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China|Univ Chinese Acad Sci Beijing 100039 Peoples R China;

    Wuxi Environm Monitoring Ctr Wuxi 214121 Jiangsu Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Sci Inst Hydrobiol State Key Lab Freshwater Ecol & Biotechnol Key Lab Algal Biol Wuhan 430072 Hubei Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Microcystis; Dolichospermum; Succession; Phosphorus limitation; Phosphatase; Polyphosphate;

    机译：微囊囊;Dolichospermum;连续;磷限制;磷酸酶;多磷酸盐;

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