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首页> 外文期刊>Harmful Algae >Comparative transcriptomics of toxin synthesis genes between the non-toxin producing dinoflagellate Cochlodiniun polykrikoides and toxigenic Alexandrium pacificum
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Comparative transcriptomics of toxin synthesis genes between the non-toxin producing dinoflagellate Cochlodiniun polykrikoides and toxigenic Alexandrium pacificum

机译：非毒素生产Dinoflagelate Cochlodiniun Polykrikoides和Toxigenic Alexandrium Pacifumum之间的比较转录组织毒素合成基因

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摘要

In the present study, we extensively characterized potential toxin-related genes, including polyketide synthase (PKS), saxitoxin (STX) and fatty acid synthase (FAS) from the non-toxin producing marine dinoflagellate Cochlodinium polykrikoides, comparing to those of a toxigenic dinoflagellate Alexandrium pacificum. RNA sequencing revealed 50 and 271 PKS contigs from C. polykrikoides and A. pacificum, respectively. According to domain constitute and amino acid alteration, we further classified the dinoflagellate type I PKS genes into 4 subgroups. Type III PKS was first identified in C. polykrikoides. Interestingly, we detected a large number (242 and 288) of homologs of 18 sxt genes from two studied dinoflagellates. Most of the eight key genes (sxtA, sxtB, sxtD, sxtG, sxtH/T, sxtI, sxtS and sxtU) for STX synthesis were detected in both dinoflatellates, whereas a core STX biosynthesis gene sxtG was not detected in C. polykrikoides. This may partially explain the absence of saxitoxin production in C. polykrikoides. In addition, we identified several type I and type II FAS genes, including FabD, FabF, FabG, FabH, FabI, and FabZ, whereas FabB was not found in C. polykrikoides. Overall, the numbers of the toxin-related genes in C. polykrikoides were less than that of A. pacificum. Phylogenetic analyses showed that type I PKS/FASs of dinoflagellates had close relationships with apicomplexans and bacteria. These suggest that the toxin-related PKS and sxt genes are commonly present in toxigenic and non-toxin producing dinoflagellates, and may be involved not only in the toxin synthesis, but also in other related molecular metabolic functions.
机译：在本研究中，我们广泛地表征了潜在的毒素相关基因，包括来自非毒素生产海洋恐龙钴酸核苷酸核酸核苷酸的非毒素生产船舶的聚酮合成酶（PKS），Saxitoxin（STX）和脂肪酸合成酶（FAS），与毒素丁氏素物相比Alexandrium Pacificum。 RNA测序分别显示来自C.Polykrikoides和A.pacificum的50％和271 pks Contigs。根据结构域构成和氨基酸改变，我们进一步将Dinoflagellate I型PKS基因分类为4个亚组。第III型PKS首先在C. polykrikoides中鉴定。有趣的是，我们检测到来自两个研究的丁曲素物的18个SXT基因的大量（242和288）。在DinoFlateLates中检测到STX合成的八个关键基因（SXTA，SXTB，SXTD，SXTG，SXTH / T，SXTS，SXTS和SXTS和SXTS和SXTS和SXTS和SXTS和SXTS和SXTS和SXTS和SXTU，SXTS和SXTS和SXTS和SXTS和SXTS和SXTU，SXTS和SXTS和SXTI）。在C.PolyKrikoides中未检测到核心STX生物合成基因SXTG。这可能部分解释在C.Polykrikoides中没有苏克替毒素的产生。此外，我们确定了几种I型和II型FAS基因，包括Fabd，Fabf，Fabg，Fabh，Fabi和Fabz，而Fabb未在C.PolyKrikoides中找到。总的来说，C.Polykrikoides中的毒素相关基因的数量小于A. Pacififum的数量。系统发育分析表明，Dinoflagellates的I型PKS /小组与ApiCoMemplex人和细菌具有密切的关系。这些表明毒素相关的PK和SXT基因通常存在于毒素和非毒素中产生恐龙，并且不仅可以在毒素合成中涉及，而且可以涉及其他相关的分子代谢功能。

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  • 来源
    《Harmful Algae》 |2020年第3期|101777.1-101777.13|共13页
  • 作者

    Wang Hui; Guo Ruoyu; Lim Weol-Ae; Allen Andrew E.; Ki Jang-Seu;

  • 作者单位

    Sangmyung Univ Dept Biotechnol Seoul 03016 South Korea;

    Sangmyung Univ Dept Biotechnol Seoul 03016 South Korea|State Ocean Adm Key Lab Marine Ecosyst & Biogeochem Beijing Peoples R China|Minist Nat Resources Inst Oceanog 2 Beijing Peoples R China;

    NIFS Ocean Climate & Ecol Res Div Busan 46083 South Korea;

    Univ Calif San Diego Scripps Inst Oceanog La Jolla CA 92093 USA|J Craig Venter Inst Microbial & Environm Genom Grp La Jolla CA 92037 USA;

    Sangmyung Univ Dept Biotechnol Seoul 03016 South Korea;

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  • 正文语种 eng
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  • 关键词

    Cochlodinium polykrikoides; Alexandrium pacificum; Transcriptome; Polyketide synthase; Saxitoxin; Fatty acid synthase;

    机译：Cochlodinium polykrikoides;Alexandrium pacificum;转录组;聚酮合成酶;萨克替毒素;脂肪酸合成酶;

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