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首页> 外文期刊>Harmful Algae >Toxic effects of the dinoflagellate Karenia mikimotoi on zebrafish (Danio rerio) larval behavior
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Toxic effects of the dinoflagellate Karenia mikimotoi on zebrafish (Danio rerio) larval behavior

机译：Dinoflagellate Karenia Mikimotoi对斑马鱼（Danio Rerio）幼虫行为的毒性作用

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摘要

Karenia mikimotoi is a toxic dinoflagellate that forms harmful blooms in coastal waters, threatening aquaculture worldwide. However, we do not know whether K. mikimotoi has a neurotoxic effect on aquatic animal behavior. Thus, this study investigated potential K. mikimotoi neurotoxicity in zebrafish larvae. Cells of K. mikimotoi were collected at the mid-exponential phase from a batch culture to prepare ruptured cell solutions (RCS). At 6 h postfertilization (hpf), zebrafish embryos were exposed to different RCS concentrations (0, 102, 103, 104, and 2.5 x 104 cells mL-1). After 120 hpf, treated larvae were collected to analyze locomotor behavior; activities of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT); and expression of genes related to neurodevelopment. We found that RCS did not affect survival rate, but significantly decreased larval locomotion, as well as their AChE, SOD, and CAT activity. Additionally, the examination of the day-night behavioral experiment revealed RCS decreased locomotion only at night. Zebrafish larvae were also significantly hypoactive in response to light and sound stimulations. Of the neurodevelopment genes, three (th, neurog1, and neurod1) were downregulated, while two (bdnf and manf) were upregulated. Our study suggests that K. mikimotoi neurotoxicity occurs through causing oxidative damage, as well as disorders in the cholinergic system and nervous system development. The results provide new insight that K. mikimotoi in low abundance did not cause significant lethal effect but still exhibited significant neurotoxicity on aquatic animals.
机译：Karenia Mikimotoi是一种有毒的丁食物恐龙，在沿海水域形成有害的盛开，威胁到全球水产养殖。然而，我们不知道K.Mikimotoi是否对水生动物行为具有神经毒性作用。因此，本研究研究了斑马鱼幼虫中的潜在的K.Mikimotoi神经毒性。从分批培养物中收集K.MikimotoI的细胞以制备破裂的细胞溶液（RCS）。在6小时内溶解（HPF），斑马鱼胚胎暴露于不同的RCS浓度（0,102,103,104和2.5×10 4个细胞ML-1）。 120 HPF后，收集处理的幼虫以分析机车行为;乙酰胆碱酯酶（疼痛），超氧化物歧化酶（SOD），过氧化氢酶（猫）的活性;与神经发育相关的基因的表达。我们发现RCS没有影响生存率，但大幅下降，幼虫运动，以及它们的疼痛，草皮和猫活动。此外，昼夜行为实验的检查揭示了RCS仅在夜间减少了运动量。斑马鱼幼虫响应光和声刺激也显着低吸引力。在神经发育基因中，下调三（Th，Neurog1和Neurod1），而两个（BDNF和MANF）被上调。我们的研究表明，K.Mikimotoi神经毒性通过导致氧化损伤以及胆碱能系统和神经系统发育中的疾病发生。结果提供了新的见解，即低丰度的K.Mikimotoi没有引起显着的致命作用，但在水生动物上仍然表现出显着的神经毒性。

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  • 来源
    《Harmful Algae》 |2021年第3期|101996.1-101996.11|共11页
  • 作者

    Niu Xiaoqin; Xu Shengnan; Yang Qiongying; Xu Xuelian; Zheng Miaomiao; Li Xi; Guan Wanchun;

  • 作者单位

    Wenzhou Med Univ Sch Lab Med & Life Sci Wenzhou Key Lab Sanit Microbiol Key Lab Lab Med Minist Educ Wenzhou 325035 Zhejiang Peoples R China;

    Wenzhou Med Univ Affiliated Kangning Hosp Wenzhou 325035 Zhejiang Peoples R China;

    Wenzhou Med Univ Sch Lab Med & Life Sci Wenzhou Key Lab Sanit Microbiol Key Lab Lab Med Minist Educ Wenzhou 325035 Zhejiang Peoples R China;

    Wenzhou Med Univ Sch Lab Med & Life Sci Wenzhou Key Lab Sanit Microbiol Key Lab Lab Med Minist Educ Wenzhou 325035 Zhejiang Peoples R China;

    Wenzhou Med Univ Affiliated Kangning Hosp Wenzhou 325035 Zhejiang Peoples R China;

    Wenzhou Med Univ Affiliated Kangning Hosp Wenzhou 325035 Zhejiang Peoples R China;

    Wenzhou Med Univ Sch Lab Med & Life Sci Wenzhou Key Lab Sanit Microbiol Key Lab Lab Med Minist Educ Wenzhou 325035 Zhejiang Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Animal behavior; Dinoflagellate; Karenia mikimotoi; Neurotoxicity; Zebrafish larvae;

    机译：动物行为;Dinoflagellate;Karenia mikimotoi;神经毒性;斑马鱼幼虫;

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