Construction of Reshaping Single-domain Antibody against CD3

Liu Xifu; Gu Zheng; Xiao Sa; Wang Yong; Zhang Weiguo; Chen Ai; Lin Qing; Huang Hualiang; Sun Jian; Chen Runsheng

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Construction of Reshaping Single-domain Antibody against CD3

机译：重塑针对CD3的单域抗体的构建

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摘要

The combining site of OKT3 was modeled, and Human Ig LS1 and ND were selected as acceptors of CDRs of OKT3 VL and VH to construct a reshaping antibody against CD3. By comparing OKT3, LS1 and ND, with their own family sequences, some residues were changed and the reshaping VL and VH genes were designed. The full VH gene was assembled in three steps with eight chemically synthesized oligonucleotide fragments using overlapping PCR and sequenced. The VH gene was expressed as active protein and inclusion bodies in the vectors of pCOMB3 and pGEX-4T-1 by ELISA and Western blot analysis.

机译：对OKT3的结合位点进行建模，并选择人Ig LS1和ND作为OKT3 VL和VH的CDR的受体，以构建针对CD3的重塑抗体。通过比较OKT3，LS1和ND及其各自的家族序列，改变了一些残基，并设计了重塑VL和VH基因。使用重叠PCR将完整的VH基因与八个化学合成的寡核苷酸片段分为三个步骤进行组装和测序。通过ELISA和Western印迹分析，VH基因在pCOMB3和pGEX-4T-1载体中表达为活性蛋白和包涵体。

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来源
《High Technology Letters》 |1996年第1期|p.107-110|共4页
作者
Liu Xifu; Gu Zheng; Xiao Sa; Wang Yong; Zhang Weiguo; Chen Ai; Lin Qing; Huang Hualiang; Sun Jian; Chen Runsheng;
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作者单位

Institute of Genetics, Academia Sinica, Beijing 100101, P.R. China;

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原文格式 PDF
正文语种 eng
中图分类工业技术;自然科学总论;
关键词
molecular modeling; gene construction and expression; reshaping antibody; glutathione s-transferase (GST);

机译：分子建模;基因构建与表达;重塑抗体;谷胱甘肽S-转移酶（GST）;

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专利

1. Construction of expression vectors and study on single-chain antibody and reshaping single-domain antibody against CD3 [J] . LIU Xifu, XIAO Sa, GU Zheng, Science in China, Series C. Life science . 1997,第3期

机译：表达载体的构建及针对CD3的单链抗体和重塑单域抗体的研究
2. Construction of Reshaping Single-domain Antibody against CD3 [J] . 刘喜富高技术通讯：英文版 . 1996,第001期

机译：对CD3重塑单结构域抗体的构建
3. Construction of a Semisynthetic Human VH Single-Domain Antibody Library and Selection of Domain Antibodies against -Crystalline of Mycobacterium tuberculosis [J] . Bahara Nur Hidayah Hairul, Chin Siang Tean, Choong Yee Siew, Journal of biomolecular screening: The official journal of the Society for Biomolecular Screening . 2016,第1期

机译：半合成人VH单域抗体文库的构建及结核分枝杆菌结晶的结构域抗体的选择
4. Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays [C] . Alyona Sukhanova, Fernanda Ramos-Gomes, Patrick Chames, International Conference on Applications of Optics and Photonics . 2019

机译：基于纳米颗粒的缀合物的纳米光影工具与单结构域抗体进行多光子微转移检测和超声生物化学测定
5. Comparative pharmacology of the human CD19/CD3 Bispecific T-cell engager (BiTERTM) antibody blinatumomab with the hybrid murine surrogate CD19/CD3 BiTE antibody hyS103. [D] . Fuhrmann, Stacy R. 2010

机译：人CD19 / CD3双特异性T细胞衔接子（BiTERTM）抗体blinatumomab与杂种鼠替代CD19 / CD3 BiTE抗体hyS103的比较药理学。
6. Construction of a reshaped HMFG1 antibody and comparison of its fine specificity with that of the parent mouse antibody. [O] . M E Verhoeyen, J A Saunders, M R Price, 1993

机译：重组HMFG1抗体的构建及其与亲本小鼠抗体的特异性比较。
7. Construction of a Semisynthetic Human VH Single-Domain Antibody Library and Selection of Domain Antibodies against α-Crystalline of Mycobacterium tuberculosis [O] . Nur Hidayah Hairul Bahara, Siang Tean Chin, Yee Siew Choong, 2015

机译：半合成人VH单结构域抗体文库的构建及其对α结晶α结晶的域抗体的选择

Construction of Reshaping Single-domain Antibody against CD3

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