PEG Delivery of a Wheat Storage Protein Gene into Rice Protoplasts

An Daochang; M. R. Davey; E.C. Cocking

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PEG Delivery of a Wheat Storage Protein Gene into Rice Protoplasts

机译：PEG将小麦贮藏蛋白基因导入水稻原生质体

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An effiecent cell suspension culture system has been initiated using callus obtained from mature seeds of the Chinese Japonica variety Eryi 105 as a source. Over the last 12 months, using the "Nottingham Method", protoplasts have been produced from these cell suspensions , and via the PEG method of transformation. 45 regenerated plants were obtained from DNA-treated protoplasts selected by 80μg/ml G418. The research of molecular analysis on those plantlets is being carried on by Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham, U. K.

机译：高效的细胞悬浮培养系统已经启动，其使用的是从中国粳稻品种Eryi 105的成熟种子中获得的愈伤组织。在过去的12个月中，使用“诺丁汉法”（Nottingham Method），已从这些细胞悬液中并通过PEG转化法生产了原生质体。从经80μg/ ml G418选择的DNA处理原生质体中获得45株再生植物。英国诺丁汉大学生命科学系植物遗传操作小组正在对这些幼苗进行分子分析研究。

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《High Technology Letters》 |1995年第1期|p.18-21|共4页
作者
An Daochang; M. R. Davey; E.C. Cocking;
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作者单位

China National Center for Biotechnology Development, Beijing 100081, P.R.China;

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原文格式 PDF
正文语种 eng
中图分类工业技术;自然科学总论;
关键词
PEG delivery; gene; rice protoplast;

机译：PEG传递;基因;水稻原生质体;

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专利

1. PEG Delivery of a Wheat Storage Protein Gene into Rice Protoplasts [J] . 高技术通讯：英文版 . 1995,第001期

机译：PEG将小麦储存蛋白基因递送到水稻原生质体中
2. Effects of various promoter/exon/intron combinations on foreign gene expression and selection efficiency following PEG-mediated transformation of einkorn wheat protoplasts. [J] . Guo GQ Russian Journal of Plant Physiology . 2000,第3期

机译：PEG介导的小麦单质原生质体转化后，各种启动子/外显子/内含子组合对外源基因表达和选择效率的影响。
3. A Novel Co-immunoprecipitation Protocol Based on Protoplast Transient Gene Expression for Studying Protein-protein Interactions in Rice [J] . Yang Jia Wei, Fu Jia Xin, Li Jing, Plant molecular biology reporter . 2014,第1期

机译：基于原生质体瞬时基因表达的新型共免疫沉淀方案用于研究水稻中的蛋白质相互作用
4. Trafficking pathway of wheat storage proteins in transgenic rice endosperm [C] . M.Oszvald, P.Tosi, L.Tam(a)s, International gluten workshop;Ministry of Agriculture;Ministry of Science and Technology;Studies of Wheat Agronomic Traits and Varietal Improvement . 2012

机译：小麦贮藏蛋白在转基因水稻胚乳中的运输途径
5. Promoter analysis of a rice seed storage protein glutelin gene and expression of bean 7S seed storage protein beta-phaseolin in transgenic rice plants. [D] . Zheng, Zhenwei. 1994

机译：水稻种子贮藏蛋白谷蛋白基因的启动子分析和转基因水稻植株7S种子贮藏蛋白β-菜豆蛋白的表达。
6. Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum [O] . Tsai-Mei Ou-Lee, Robert Turgeon, Ray Wu 2015

机译：电穿孔水稻，小麦和高粱的原生质体后，与植物病毒或果蝇启动子连接的外源基因的表达
7. PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts [O] . Rodrigo Ribeiro Arnt Sant’Ana, Clarissa Alves Caprestano, Rubens Onofre Nodari, 2020

机译：PEG递送CRISPR-CAS9核糖核蛋白系统，用于玉米原生质体的基因编辑筛选

PEG Delivery of a Wheat Storage Protein Gene into Rice Protoplasts

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