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Three-dimensional Structure of Rice Dwarf Virus by Electron Cryomicroscopy

机译：电子低温显微镜观察稻矮病毒的三维结构

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摘要

The three-dimensional (3D) structure of intact rice dwarf virus (RDV) has been determined to 2.6 nm resolution by 400kV electron cryomicroscopy and computer reconstruction techniques. The intact virion of 69.8 nm in diameter was seen to be made up of an outer shell of 6.9 nm in thickness and an inner shell of 2.5 nm in thickness at a diameter of 54 nm. The outer shell surface forms a T = 13 icosahedral lattice with holes at 5- and 6-coordinated positions. The 780 capsomeres at local 3-fold positions appear trimeric. The interface between these two shells could be clearly delineated and the interaction was maintained by a cylindrical density whose upper domain connects subunits of the trimeric capsomeres by an interlocking mechanism. The 3D structure of inner shell was also reconstructed to 3.3 nm from 100kV images of purified inner shell particles, confirming our interpretation of the multilayer structure of intact RDV.
机译：完整的稻矮病毒（RDV）的三维（3D）结构已通过400kV电子冷冻显微镜和计算机重建技术确定为2.6 nm分辨率。可以看到直径为69.8 nm的完整病毒体由厚度为6.9 nm的外壳和直径为54 nm的厚度为2.5 nm的内壳组成。外壳表面形成T = 13二十面体晶格，在5和6坐标位置处有孔。在局部3倍位置的780衣壳呈现三聚体。可以清楚地描绘出这两个壳之间的界面，并通过圆柱密度保持相互作用，该圆柱密度的上部结构域通过互锁机制连接三聚体脂质体的亚基。内壳的3D结构也可以从纯化的内壳颗粒的100kV图像重建到3.3 nm，这证实了我们对完整RDV多层结构的解释。

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