Rabbit Defensin (NP-1) Gene Transformation of Wheat and In Vitro Microbicidal Activity Assay of Transgenic Plants

Guo Dianjing; Fu Rongzhao; Li Wenbin

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Rabbit Defensin (NP-1) Gene Transformation of Wheat and In Vitro Microbicidal Activity Assay of Transgenic Plants

机译：小麦的兔防卫素（NP-1）基因转化和转基因植物的体外杀微生物活性测定

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Monocot high expression vector pBARUNP1, harboring rabbit defensin (NP-1) gene and selective bar gene for resistance to the herbicide Basta, were constructed and then trans- ferred into immature embryos of wheat ("Bobwhite" and "Zhong 60634") via particle bom- bardment. Southern and RNA dot blots showed the stable integration and transcription of foreign NP-1 gene in the wheat genome. Furthermore, in vitro microbicidal activity assay in- dicated the proper translation of defensin. Crude protein extraction of transgenic plants ex- hibited to some extent cytotoxic to several pathogens including G. saubinetii, B. subtilis, E. coli, and A. tumefaciens.

机译：构建了具有兔防卫素（NP-1）基因和选择性除草剂Basta抗性的bar基因的单子叶植物高表达载体pBARUNP1，然后通过颗粒将其转移到小麦的未成熟胚中（“ Bobwhite”和“ Zhong 60634”）轰击。 Southern和RNA斑点印迹显示外源NP-1基因在小麦基因组中稳定整合和转录。此外，体外杀微生物活性测定表明防御素的正确翻译。转基因植物的粗蛋白提取物在一定程度上对几种病原体具有细胞毒性，这些病原体包括沙门氏菌，枯草芽孢杆菌，大肠杆菌和根癌农杆菌。

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来源
《High Technology Letters》 |1999年第1期|p.95-98|共4页
作者
Guo Dianjing; Fu Rongzhao; Li Wenbin;
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正文语种 eng
中图分类工业技术;
关键词
Wheat; Rabbit defensin (NP-1) gene; Transgenic plant;

机译：小麦;兔防御素（NP-1）基因;转基因植物;

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1. Rabbit Defensin(NP-1) Gene Transformation of Wheat and In Vitro Microbicidal Activity Assay of Transgenic Plants [J] . 郭殿京高技术通讯：英文版 . 1999,第001期

机译：转基因植物的小麦和体外微生物活性测定的兔Defensin（NP-1）基因转化
2. Rabbit defensin (NP-1) genetic engineering of plant [J] . Zhou Wen Ting, Hao Ming Ming, Liu Jing, African Journal of Biotechnology . 2011,第46期

机译：兔Defensin（NP-1）植物基因工程
3. Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa) [J] . D. Song, X. Xiong, W.F. Tu, Genetics and Molecular Research . 2017,第1期

机译：兔防御素NP-1基因在莴苣中的转移与表达
4. Studies on expression of rabbit defensin gene (NP-1) in transgenic tobacco [C] . Qiang Han, Jing Liu, Wei Yao, International Conference on Medical Engineering and Bioinformatics . 2015

机译：转基因烟草中兔Defensin基因（NP-1）表达的研究
5. Genetic transformation of Micro-Tom tomato with a calcium signal modifying gene and analysis of transgenic plants challenged with ' Candidatus Liberibacter solanacearum'. [D] . Lott, Cecilia C. M. 2014

机译：Micro-Tom番茄具有钙信号修饰基因的遗传转化和对'Candidatus Liberibacter solanacearum'攻击的转基因植物的分析。
6. Fungicidal properties of defensin NP-1 and activity against Cryptococcus neoformans in vitro. [O] . M S Alcouloumre, M A Ghannoum, A S Ibrahim, 1993

机译：防御素NP-1的杀真菌特性和体外对新型隐球菌的活性。
7. Rabbit defensin (NP-1) genetic engineering of plant [O] . Wen Ting Zhou, Ming Ming Hao, Jing Liu, 2011

机译：兔Defensin（NP-1）植物基因工程
8. Value of the Indirect Immunoradiometric Assay of Serum alpha - Fetoprotein in Detecting Liver Regeneration and Neoplastic Transformation in Chronic Liver Disease. Part of a Coordinated Programme on in Vitro Assay Techniques. Final [R] . Voiculetz, N. 1979

机译：血清甲胎蛋白间接免疫放射分析法检测慢性肝病肝再生和肿瘤转化的价值。体外分析技术协调计划的一部分。最后

Rabbit Defensin (NP-1) Gene Transformation of Wheat and In Vitro Microbicidal Activity Assay of Transgenic Plants

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