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From Thoreau's woods to the Canary Islands: exploring ocean biogeochemistry through enzymology

机译:从梭罗的树林到加那利群岛:通过酶学探索海洋生物地球化学

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This essay relates my odyssey in exploring enzyme reactions as oceanographic rate proxies and describes my scientific contributions since 1963. To elucidate biogeochemical processes in marine ecosystems I explored calculating respiratory oxygen utilization (OUR) and nitrate respiration from activities of the respiratory electron transport system (ETS), assimilatory phytoplankton nitrate uptake from nitrate reductase activity, and respiratory CO2 production from isocitrate dehydrogenase. This exploration began at Woods Hole Oceanographic Institute doing a thesis on Krebs-Cycle-based respiration in the quahog, Venus mercenaria, for my B. Sc. at the Massachusetts Institute of Technology (MIT). It continued at the Friday Harbor Marine Laboratory (FHL) of the University of Washington (UW) developing a biological oceanography MS thesis testing succinate dehydrogenase activity as a respiration proxy in Artemia salina. Upon realizing that the ETS, not the Krebs-Cycle, controlled the electron flux to O2, I developed the ETS idea to determine seawater OUR for a Ph. D. thesis at UW. The resulting assay led to the first direct measurements of deep-sea metabolism and allowed biochemical calculations of OUR profiles in the Costa Rica Dome, in the Peru upwelling, and in other ocean water columns. I continued this research at Maine's Bigelow Laboratory for Ocean Science (BLOS), and at Quebec's Institute Maurice Lamontagne (IML). Then, after moving to Spain, I used the stability of my pension to continue this research at the University of Las Palmas de Gran Canaria (ULPGC) where I am catalysing new thinking about ocean metabolism. Here, these topics are integrated into an autobiographic history of this science.
机译:本文涉及我在探索作为海洋学速率代理的酶反应方面的冒险经历,并描述了我自1963年以来的科学贡献。 ),硝酸盐还原酶活性吸收同化浮游植物的硝酸盐以及异柠檬酸脱氢酶产生的呼吸道二氧化碳。这项探索始于伍兹霍尔海洋研究所,他为我的理学士学位论文撰写了有关克雷布斯周期的呼吸作用,该研究在Quhog的金星上发生。在麻省理工学院(MIT)。它继续在华盛顿大学(UW)的星期五港口海洋实验室(FHL)进行,研究了生物海洋学MS论文,测试了琥珀酸脱氢酶活性作为盐卤中呼吸替代的活性。在意识到ETS而不是克雷布斯循环控制了电子向O2的通量后,我提出了ETS的想法来确定UW的博士学位论文的海水OUR。所得测定法导致了对深海代谢的首次直接测量,并允许对哥斯达黎加圆顶,秘鲁上升流以及其他海水柱中的OUR剖面进行生化计算。我在缅因州比格洛海洋科学实验室(BLOS)和魁北克莫里斯·拉蒙塔涅研究所(IML)进行了这项研究。然后,在移居西班牙后,我利用退休金的稳定性在大加那利岛拉斯帕尔马斯大学(ULPGC)继续进行这项研究,在那里我激发了有关海洋代谢的新思维。在这里,这些主题被整合到该科学的自传历史中。

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