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首页> 外文期刊>ICES Journal of Marine Science >Ageing octopods from stylets: development of a technique for permanent preparations
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Ageing octopods from stylets: development of a technique for permanent preparations

机译：探针中的章鱼变老：永久性制备技术的发展

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Previous attempts at ageing octopods from stylets have relied on preparations that deteriorate with time. Some techniques require an immediate photographic record, others allow real-time enumeration but do not provide a permanent archive. A technique is described that produces permanent and archivable preparations of octopod stylets. Stylets were dehydrated in ethanol and infiltrated with a low-viscosity resin. Subsequent polymerization of the resin allowed the embedded stylet to be ground and polished to reveal the stylet microstructure. This comprised increments that are probably suitable for age estimation. The technique was developed using stylets of Octopus vulgaris and Eledone cirrhosa. Increments were composed of light and dark bands and were clearly denned at × 400 and at ×625 magnifications. The number of increments ranged from 189 to 399. The stylets of a deep-sea species (Bathypolypus sponsalis) and an Antarctic species (Megaleledone setebos) were also examined. Each appeared to have growth increments, despite the perception that the environments they inhabited may not provide daily cues. Using the technique developed, the pre-hatch nucleus was seldom well denned, as reported for O. pallidus, stylets of which were prepared using a non-permanent method. Reasons for this are discussed. The microstructure clarity revealed is probably associated with the ultra-low viscosity of the resin used.
机译：以前尝试从管心针老化章鱼的方法是依靠随时间而恶化的制剂。有些技术需要即时的摄影记录，而另一些则允许实时枚举，但不提供永久存档。描述了一种产生章鱼探针永久性和可归档制剂的技术。探针在乙醇中脱水，并用低粘度树脂渗透。树脂随后的聚合反应允许对嵌入的管心针进行研磨和抛光，以显示管心针的微观结构。这包括可能适合年龄估计的增量。该技术是用八达通和Eledone cirrhosa的探针开发的。增量由亮带和暗带组成，并且在×400和×625放大倍率下清晰地确定。增量的数量范围为189至399。还检查了深海物种（Bathypolypus sponsalis）和南极物种（Megaleledone setebos）的探针。尽管人们认为他们所居住的环境可能无法提供日常线索，但每个人似乎都有增长。使用已开发的技术，孵化前的细胞核很少得到良好的染色，如关于苍鹭的报道，该鹅的探针使用非永久性方法制备。讨论了其原因。揭示的微观结构清晰度可能与所用树脂的超低粘度有关。

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