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首页> 外文期刊>ICES Journal of Marine Science >Transgenerational marking of cephalopods with an enriched barium isotope: a promising tool for empirically estimating post-hatching movement and population connectivity
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Transgenerational marking of cephalopods with an enriched barium isotope: a promising tool for empirically estimating post-hatching movement and population connectivity

机译:富含钡同位素的头足类的世代标记:凭经验估计孵化后移动和种群连通性的有前途的工具

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摘要

Quantifying the movement of very small and young individuals, determining sources of recruitment, and identifying the contribution of populations from different regions and periods to fished stocks is a major ecological challenge. Transgenerational isotope labelling (TRAIL), a technique which enables offspring to be marked on a mass scale, is applied for the first time to cephalopods, facilitating field studies quantifying population connectivity. Four species were used: Sepioteuthis australis, Euprymna tasmanica, Octopus pallidus, and Octopus maorum. Gravid females were injected with the enriched stable isotope ~(137)Ba in different body tissues at several different doses. Isotopic ratios ~(138)Ba:~(137)Ba were then quantified using laser ablation inductively coupled plasma-mass spectrometry on the hard structures (statoliths and stylets) of offspring produced by the injected females. Day-old hatchlings from both squid species had statoliths with isotopic ratios significantly different from natural ratios and control animals, but variability in the ratios in hatchlings produced by different females was independent of dose or injection location. No differences were observed in the statoliths and stylets removed from hatchlings and juveniles, respectively, from the two octopus species, although isotopic shifts were evident in the hard structures of the adults injected. The use of TRAIL is a technique that offers considerable potential to advance the understanding of post-hatching dispersal and population connectivity in cephalopod populations.
机译:量化非常小的和年轻的个体的流动,确定征募来源以及确定不同地区和时期的人口对鱼类种群的贡献是一项重大的生态挑战。跨代同位素标记(TRAIL)是一种使后代得以大规模标记的技术,该技术首次应用于头足类动物,从而促进了量化种群连通性的野外研究。使用了四种:澳大利亚的Sepioteuthis australis,塔斯普里Euprymna tasmanica,苍白章鱼和大章鱼。妊娠雌性动物以不同的剂量在不同的身体组织中注入了丰富的稳定同位素〜(137)Ba。然后使用激光烧蚀电感耦合等离子体质谱法对注入雌性小鼠产生的后代硬结构(静立柱和管心针)进行同位素比〜(138)Ba:〜(137)Ba定量。两种鱿鱼种类的日龄孵化场均具有与天然比例和对照动物明显不同的同位素比,但是不同雌性所孵化的孵化场中比例的变化与剂量或注射位置无关。尽管在注射的成年动物的硬结构中同位素迁移明显,但从两种章鱼中分别从孵化场和幼鱼中取出的针状石和探针没有观察到差异。 TRAIL的使用为在头足类种群中促进孵化后扩散和种群连通性的理解提供了巨大潜力。

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