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首页> 外文期刊>Ichthyological Research >Species identification of upstream fatminnow Rhynchocypris oxycephalus and downstream fatminnow Rhynchocypris lagowskii, based on PCR-RFLP of mitochondrial DNA
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Species identification of upstream fatminnow Rhynchocypris oxycephalus and downstream fatminnow Rhynchocypris lagowskii, based on PCR-RFLP of mitochondrial DNA

机译：基于线粒体DNA的PCR-RFLP技术鉴定上游小头冬虫和下游小头冬虫

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摘要

The morphological similarity between upstream fatminnow Rhynchocypris oxycephalus and downstream fatminnow Rhynchocypris lagowskii makes it difficult to discriminate accurately between these species in rivers where they coexist. For easy and precise identification of these two species, we developed a genetic discrimination method based on PCR-RFLP analysis for specimens from the Inohzawa River watershed in the Izu Peninsula, central Honshu, Japan. This genetic method was applied to the species identification of the fatminnows from two other watersheds, the Kano and Kawazu Rivers, flowing across the peninsula from north to south. We present the genetic evidence for the restricted distribution of R. oxycephalus and the ubiquitous distribution of R. lagowskii in the peninsula.
机译：上游fat鱼Rhynchocycys oxycephalus和下游fat鱼Rhynchocycys lagowskii之间的形态相似性使得很难在它们共存的河流中准确地区分这些物种。为了轻松，准确地识别这两个物种，我们开发了一种基于PCR-RFLP分析的遗传判别方法，用于对日本本州中部伊豆半岛伊野泽河流域的标本进行分析。这种遗传方法被用于识别另外两个流域（从卡诺河和卡瓦祖河（Kano and Kawazu Rivers），从北到南流经该半岛）的min鱼的物种。我们提出的遗传证据为R. oxycephalus的限制分布和R. lagowskii在半岛的普遍分布。

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  • 来源
    《Ichthyological Research》 |2012年第2期|p.156-163|共8页
  • 作者

    Noriyuki Takai; Yuta Abiko; Harumi Tsukamoto; Ai Miura; Kohei Yuasa; Shiro Itoi; Shizuko Nakai; Haruo Sugita; Kiyoshi Yoshihara;

  • 作者单位

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, 252-0880, Japan;

    Department of Marine Science and Resources, Colle;

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  • 正文语种 eng
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  • 关键词

    Rhynchocypris; Fatminnow; Species identification; PCR–RFLP; Izu Peninsula;

    机译：豚鼠;Fatminnow;种属鉴定;PCR-RFLP;伊豆半岛;

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