• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IEE Proceedings. Part M, Nanobiotechnology >Force-velocity relationship of single actin filament interacting with immobilised myosin measured by electromagnetic technique
【24h】

Force-velocity relationship of single actin filament interacting with immobilised myosin measured by electromagnetic technique

机译：电磁法测量单肌动蛋白丝与固定的肌球蛋白相互作用的力-速关系

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of applying an external load to actin filaments moving in the in vitro motility assay is studied. Bead-tailed actin filaments were made by polymerising actin onto 2.8 μm diameter Dynabeads conjugated with gelsolin-G actin. These were introduced into a motility cell coated with 100μg/ml rabbit fast skeletal myosin in the presence of ATP and 0.5% methylcellulose. The motility cell was inserted between the pole-pieces of an electromagnet and the fluorescent beads and filaments were observed. The force-current relationship of the electromagnet was determined from the velocity of free beads in viscous solution and Stokes' equation. The magnet produced up to 6pN force on the Dynabeads at 1 A. Many bead-tailed actin filaments stuck to the surface, but the beads that did move moved at the same speed as unloaded f-actin in the same cell. Bead-tailed filaments slowed down under an increasing magnetic load, eventually stalled and then slid backward under increasing load before detaching from the surface. Single-filament force-velocity curves were constructed and a stalling force of about 0.6 pN/mm of actin filament estimated.
机译：研究了在体外运动测定中对肌动蛋白丝施加外部载​​荷的效果。通过将肌动蛋白聚合到与凝溶胶蛋白-G肌动蛋白缀合的2.8μm直径的Dynabeads上，制得珠尾肌动蛋白丝。在存在ATP和0.5％甲基纤维素的情况下，将它们引入涂有100μg/ ml兔快速骨骼肌球蛋白的运动细胞中。将运动细胞插入电磁体的极靴之间，观察到荧光珠和细丝。电磁力的力-电流关系由粘性溶液中自由磁珠的速度和斯托克斯方程确定。磁铁在1 A电流下对Dynabeads产生高达6pN的力。许多珠尾肌动蛋白丝附着在表面上，但确实移动的珠子与在同一单元中加载的f-肌动蛋白的移动速度相同。珠尾长丝在增加的磁性负载下减速，最终失速，然后在增加的负载下向后滑动，然后从表面分离。绘制单丝力-速度曲线，估计肌动蛋白丝的失速力约为0.6 pN / mm。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号