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首页> 外文期刊>IEE Proceedings. Part M, Nanobiotechnology >Controlling cell destruction using dielectrophoretic forces
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Controlling cell destruction using dielectrophoretic forces

机译：使用介电泳力控制细胞破坏

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摘要

Measurements are reported of the main factors, namely the AC voltage frequency and magnitude, that were observed to influence the number of cells destroyed during dielectrophoresis (DEP) experiments on Jurkat T cells and HL60 leukemia cells. Microelectrodes of interdigitated and quadrupolar geometries were used. A field-frequency window has been identified that should be either avoided or utilised, depending on whether or not cell damage is to be minimised or is a desired objective. The width and location of this frequency window depends on the cell type, as defined by cell size, morphology and dielectric properties, and is bounded by two characteristic frequencies. These frequencies are the DEP cross-over frequency, where a cell makes the transition from negative to positive DEP, and a frequency determined by the time constant that controls the frequency dependence of the field induced across the cell membrane. When operating in this frequency window, and for the microelectrode designs used in this work, cell destruction can be minimised by ensuring that cells are not directed by positive DEP to electrode edges where fields exceeding 30-40 kV/m are generated. Alternatively, this field-frequency window can be exploited to selectively destroy specific cell types in a cell mixture.
机译：据报道测量了主要因素，即交流电压频率和幅度，这些因素影响到在Jurkat T细胞和HL60白血病细胞的介电电泳（DEP）实验过程中破坏的细胞数量。使用指状和四极几何形状的微电极。已经确定了应避免或利用的场频窗口，这取决于细胞损伤是要被最小化还是期望的目标。该频率窗口的宽度和位置取决于单元类型，如单元大小，形态和介电特性所定义，并受两个特征频率的限制。这些频率是DEP穿越频率，在该频率下细胞从负DEP过渡到正DEP，并且是由时间常数确定的频率，该时间常数控制跨细胞膜感应的电场的频率依赖性。当在此频率窗口中操作时，对于本工作中使用的微电极设计，通过确保电池不被正DEP引导至产生超过30-40 kV / m的电场的电极边缘，可以最大程度地减少电池损坏。或者，可以利用该场频窗口来选择性破坏细胞混合物中的特定细胞类型。

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