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Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

机译:通过将光纤超连续谱成形为900至1160 nm的多峰非线性显微镜

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摘要

Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.
机译:非线性显微镜已广泛用于生物光子成像。脉冲整形可控制超快脉冲的非线性光学过程,以进行选择性成像和对比度增强。在这项研究中,非线性显微镜包括双光子荧光,二次谐波生成和三次谐波生成,是使用从900到1160 nm的超连续谱(SC)整形的脉冲来进行的。通过将来自Yb:KYW脉冲激光器的脉冲耦合到光子晶体光纤中而生成的SC进行了光谱过滤,并使用空间光调制器进行了压缩。成形脉冲用于细胞和组织样品的非线性光学成像。光纤SC的振幅和相位整形可在宽带宽范围内通过单光束和易于调整的设置提供选择性和有效的非线性光学成像。

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