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Longitudinal Imaging of Heart Development With Optical Coherence Tomography

机译:光学相干断层扫描对心脏发育的纵向成像

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Optical coherence tomography (OCT) has great potential for deciphering the role of mechanics in normal and abnormal heart development. OCT images tissue microstructure and blood flow deep into the tissue (1–2 mm) at high spatiotemporal resolutions allowing unprecedented images of the developing heart. Here, we review the advancement of OCT technology to image heart development and report some of our recent findings utilizing OCT imaging under environmental control for longitudinal imaging. Precise control of the environment is absolutely required in longitudinal studies that follow the growth of the embryo or studies comparing normal versus perturbed heart development to obtain meaningful in vivo results. These types of studies are essential to tease out the influence of cardiac dynamics on molecular expression and their role in the progression of congenital heart defects.
机译:光学相干断层扫描(OCT)在解释力学在正常和异常心脏发育中的作用方面具有巨大潜力。 OCT以高时空分辨率对组织的微观结构和血流深入组织(1-2mm)进行成像,从而获得了前所未有的心脏发育图像。在这里,我们回顾了OCT技术对心脏发育成像的进展,并报告了我们在环境控制下利用OCT成像进行纵向成像的最新发现。在胚胎发育后进行的纵向研究或将正常与不稳定心脏发育进行比较以获取有意义的体内结果的研究中,绝对需要精确控制环境。这些类型的研究对于弄清楚心脏动力学对分子表达的影响及其在先天性心脏缺陷进展中的作用至关重要。

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