• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Ultrasonics, Ferroelectrics and Frequency Control, IEEE Transactions on >Intravascular ultrasound detection and delivery of molecularly targeted microbubbles for gene delivery
【24h】

Intravascular ultrasound detection and delivery of molecularly targeted microbubbles for gene delivery

机译：血管内超声检测和分子靶向微泡的递送以进行基因递送

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We are investigating the combination of microbubble-based targeted drug delivery and intravascular ultrasound (IVUS) imaging as a potential therapy to reduce incidence of restenosis following stent placement in atherosclerotic coronary arteries. The goal of these studies was to determine whether IVUS could be used to detect targeted microbubbles and enhance drug/gene delivery through targeting. Quiescent vascular smooth muscle cells (SMCs) were stimulated with cytokine IL-1β to induce the inflammatory cell surface marker vascular cell adhesion molecule 1 (VCAM-1). Molecular-targeted (VCAM-1 Ab or IgG control Ab), fluorescent-labeled microbubbles were conjugated with plasmid DNA expressing green fluorescent protein (GFP, pMax-GFP) and exposed to the inflamed SMCs under flow to measure adhesion compared with control microbubbles. Gene delivery was performed using a modified IVUS catheter to generate 1.5-MHz ultrasound at 200 kPa. Detection of adherent microbubbles to inflamed SMCs in culture and flow chambers was measured using an IVUS catheter and scanner. VCAM-1-targeted microbubbles enhanced adhesion to inflamed SMCs 100-fold over nontargeted microbubbles. Compared with noninflamed SMCs, VCAM-1-targeted microbubbles exhibited a 7.9-fold increase in adhesion to IL-1β-treated cells. Targeted microbubbles resulted in a 5.5-fold increase in plasmid DNA transfection over nontargeted microbubbles in conjunction with a focused 2.54-cm (1-in) diameter 1-MHz transducer and also enhanced transfection by the modified IVUS transducer at 1.5 MHz. Targeted microbubbles (at a density of 3 × 104 microbubbles/mm2) increased IVUS image intensity 13.2 dB over non-microbubble-coated surfaces. Rupture of microbubbles from the modified IVUS transducer resulted in a 53% reduction in image intensity. Taken together, these results indicate that IVUS may be used to detect targeted microbubbles to inflamed vasculature and subsequently deliver a - ene/drug locally.
机译：我们正在研究基于微泡的靶向药物输送和血管内超声（IVUS）成像的组合，作为减少支架置入动脉粥样硬化冠状动脉后再狭窄发生率的潜在疗法。这些研究的目的是确定IVUS是否可用于检测靶向微泡并通过靶向增强药物/基因的递送。用细胞因子IL-1β刺激静止的血管平滑肌细胞（SMCs），以诱导炎性细胞表面标志物血管细胞粘附分子1（VCAM-1）。将分子靶向的（VCAM-1 Ab或IgG对照Ab）荧光标记的微泡与表达绿色荧光蛋白（GFP，pMax-GFP）的质粒DNA偶联，并在流动条件下暴露于发炎的SMC中，以与对照微泡相比测量粘附力。使用改良的IVUS导管进行基因传递，以产生200 kPa的1.5 MHz超声。使用IVUS导管和扫描仪测量培养室和流室中发炎的SMC粘附的微气泡的检测。 VCAM-1靶向微泡与发炎的SMC的粘附力比非靶向微泡高100倍。与未发炎的SMC相比，靶向VCAM-1的微泡与IL-1β处理的细胞的粘附力增加了7.9倍。与聚焦的2.54厘米（1英寸）直径1-MHz换能器结合，靶向微泡导致质粒DNA转染比非靶向微泡增加5.5倍，并且通过改良的IVUS换能器在1.5 MHz处增强了转染。靶向微泡（密度为3×10 4 微泡/ mm 2 ）在非微泡涂层表面上的IVUS图像强度增加了13.2 dB。改进的IVUS换能器破裂的微气泡导致图像强度降低53％。综上所述，这些结果表明IVUS可用于检测靶向微泡使脉管系统发炎，并随后局部递送-烯/药物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号