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Multilayer analysis: quantitative scanning acoustic microscopy for tissue characterization at a microscopic scale

机译:多层分析:定量扫描声显微镜,用于微观尺度的组织表征

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An in vitro acoustic microscopy method for the quantitative characterization of biological hard tissues at a microscopic scale is described. At a frequency of 900 MHz, the acoustic impedance is measured as a tissue parameter, which is closely related to its elastomechanical properties. Contrast influences caused by defocus, edges, and surface inclinations, respectively, are either compensated or excluded from the measurement by a special data acquisition and analysis concept. A raster grid was used to validate the capabilities and limitations of the method, and results obtained from human cortical bone are shown. The comparison of different evaluation methods demonstrate the significance of a sophisticated analysis under consideration of topographical and system parameters. Cortical bone impedance maps showed a strong dependence on the anatomical structures, and the mean values were found to be in the range from 3.5 to 6.5 Mrayl within one single osteon.
机译:描述了一种用于在微观尺度上定量表征生物硬组织的体外声学显微镜方法。在900 MHz的频率下,将声阻抗作为组织参数进行测量,这与其弹性力学特性密切相关。散焦,边缘和表面倾斜分别引起的对比度影响可以通过特殊的数据采集和分析概念来补偿或排除在测量范围之外。使用栅格网格验证了该方法的功能和局限性,并显示了从人皮层骨骼获得的结果。不同评估方法的比较表明,在考虑地形和系统参数的情况下进行复杂分析的重要性。皮质骨阻抗图显示出对解剖结构的强烈依赖性,并且发现单个骨内的平均值在3.5至6.5 Mrayl的范围内。

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