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首页> 外文期刊>Immunogenetics >Molecular characterization of coding sequences and analysis of Toll-like receptor 3 mRNA expression in water buffalo (Bubalus bubalis) and nilgai (Boselaphus tragocamelus)
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Molecular characterization of coding sequences and analysis of Toll-like receptor 3 mRNA expression in water buffalo (Bubalus bubalis) and nilgai (Boselaphus tragocamelus)

机译：水牛（Bubalus bubalis）和尼尔盖（Boselaphus tragocamelus）编码序列的分子表征和Toll样受体3 mRNA表达的分析

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摘要

Toll-like receptor 3 (TLR3), an antiviral innate immunity receptor recognizes double-stranded RNA, preferably of viral origin and induces type I interferon production, which causes maturation of phagocytes and subsequent release of chemical mediators from phagocytes against some viral infections. The present study has characterized TLR3 complementary DNA (cDNA) in buffalo (Bubalus bubalis) and nilgai (Boselaphus tragocamelus). TLR3 coding sequences of both buffalo and nilgai were amplified from cultured dendritic cell cDNA and cloned in pGEMT-easy vector for characterization by restriction endonucleases and nucleotide sequencing. Sequence analysis reveals that 2,715-bp-long TLR3 open reading frame encoding 904 amino acids in buffalo as well as nilgai is similar to that of cattle. Buffalo TLR3 has 98.6 and 97.9% identity at nucleotide level with nilgai and cattle, respectively. Likewise, buffalo TLR3 amino acids share 96.7% identity with cattle and 97.8% with nilgai. Non-synonymous substitutions exceeding synonymous substitutions indicate evolution of this receptor through positive selection among these three ruminant species. Buffalo and nilgai appear to have diverged from a common ancestor in phylogenetic analysis. Predicted protein structures of buffalo and nilgai TLR3 from deduced amino acid sequences indicate that the buffalo and nilgai TLR3 ectodomain may be more efficient in ligand binding than that of cattle. Furthermore, TLR3 messenger RNA expression in tissues as quantified by real-time PCR was found higher in nilgai than buffalo.
机译：Toll样受体3（TLR3）是一种抗病毒的先天免疫受体，可识别双链RNA（优选来自病毒）并诱导I型干扰素生成，从而导致吞噬细胞成熟并随后从吞噬细胞释放化学介质以抵抗某些病毒感染。本研究已在水牛（Bubalus bubalis）和nilgai（Boselaphus tragocamelus）中鉴定了TLR3互补DNA（cDNA）。从培养的树突状细胞cDNA中扩增出水牛和nilgai的TLR3编码序列，并将其克隆到pGEMT-easy载体中，以通过限制性核酸内切酶和核苷酸测序进行表征。序列分析表明，在水牛和黑lg中编码有904个氨基酸的2715 bp长的TLR3开放阅读框与牛相似。布法罗TLR3在核苷酸水平上与尼尔基和牛的同源性分别为98.6％和97.9％。同样，水牛TLR3氨基酸与牛具有96.7％的同一性，与尼尔基具有97.8％的同一性。超过同义替换的非同义替换表示通过在这三种反刍动物物种中的阳性选择，该受体的进化。布法罗和尼尔盖在系统发育分析中似乎与共同祖先有所不同。从推导的氨基酸序列预测的水牛和尼尔基TLR3的蛋白质结构表明，水牛和尼尔基TLR3胞外域的配体结合可能比牛更有效。此外，通过实时PCR定量分析，在尼尔基地区，组织中TLR3信使RNA的表达高于水牛。

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  • 来源
    《Immunogenetics》 |2007年第1期|69-76|共8页
  • 作者

    Animesh Dhara; Mohini Saini; Dhanjit K. Das; Devendra Swarup; Bhaskar Sharma; Satish Kumar; Praveen K. Gupta;

  • 作者单位

    Centre for Wildlife Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

    Centre for Wildlife Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

    Centre for Wildlife Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

    Centre for Wildlife Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

    Division of Biochemistry Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

    Division of Veterinary Biotechnology Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

    Division of Veterinary Biotechnology Indian Veterinary Research Institute Izatnagar Bareilly 243122 India;

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  • 正文语种 eng
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  • 关键词

    Toll-like receptor 3; Innate immunity; Bubalus bubalis; Boselaphus tragocamelus; Coding sequence;

    机译：Toll样受体3;先天免疫;野牛;牛油菌;编码顺序;

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