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首页> 外文期刊>Immunogenetics >Differential expression of two Carassius auratus Mx genes in cultured CAB cells induced by grass carp hemorrhage virus and interferon
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Differential expression of two Carassius auratus Mx genes in cultured CAB cells induced by grass carp hemorrhage virus and interferon

机译：草鱼出血病毒和干扰素诱导培养的CAB细胞中两个CA鱼Mx基因的差异表达

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摘要

UV-inactivated GCHV (grass carp hemorrhage virus) is able to induce an antiviral state in cultured CAB cells (crucian carp Carassius auratus blastulae embryonic cells) via the production of interferon (IFN). In the current work, the full-length cDNAs of two Mx genes, termed CaMx1 and CaMx2, have been cloned and sequenced from UV-inactivated GCHV-infected and still IFN-producing CAB cells by suppression subtractive hybridization. Their putative proteins show the characteristically structural features of mammalian IFN-induced Mx proteins, including GTP-binding motif, dynamin family signature and leucine zipper motif. CaMx1 exhibits 85% sequence identity to zebrafish MxA and 72–74% to three Atlantic salmon Mx proteins. CaMx2 is most similar to zebrafish MxE, with 80% identity, and then rainbow trout Mx3, with 52%. Constitutive expression was detected by RT-PCR for CaMx1, but not for CaMx2, in normal CAB cells, but their up-regulations could be induced after treatment with active GCHV, UV-inactivated GCHV and CAB IFN. Distinct kinetics of expression was observed for either CaMx1 or CaMx2 corresponding to the three stimuli, and even between CaMx1 and CaMx2, corresponding to the same stimulus. Upon virus infection, the transcriptional induction was strongly blocked for CaMx2 by cycloheximide (CHX), whereas almost nothing was observed for CaMx1. By contrast, following treatment with CAB IFN, CHX did not inhibit either gene transcription. Collectively, these results suggest that there are very distinct mechanisms for modulating the expression of both CaMx1 and CaMx2 in normal and GCHV-infected CAB cells.
机译：紫外线灭活的GCHV（草鱼出血病毒）能够通过产生干扰素（IFN）在培养的CAB细胞（cr鱼Car鱼胚细胞）中诱导抗病毒状态。在当前的工作中，已经通过抑制消减杂交从紫外线灭活的GCHV感染和仍产生IFN的CAB细胞中克隆并测序了两个Mx基因的全长cDNA，分别称为CaMx1和CaMx2。他们的推定蛋白显示出哺乳动物干扰素诱导的Mx蛋白的特征性结构特征，包括GTP结合基序，动力蛋白家族特征和亮氨酸拉链基序。 CaMx1与斑马鱼MxA具有85％的序列同一性，与三种大西洋鲑鱼Mx蛋白具有72–74％的序列同一性。 CaMx2与斑马鱼MxE最相似，具有80％的同一性，然后与虹鳟鱼Mx3具有52％的同一性。通过RT-PCR检测到正常CAB细胞中CaMx1的组成型表达，但未检测到CaMx2的组成型表达，但是在用活性GCHV，UV灭活的GCHV和CAB IFN处理后，可以诱导其上调。对于对应于三个刺激的CaMx1或CaMx2，甚至在对应于相同刺激的CaMx1和CaMx2之间，观察到了明显的表达动力学。病毒感染后，环己酰亚胺（CHX）强烈阻止了CaMx2的转录诱导，而CaMx1几乎没有观察到。相反，在用CAB IFN处理后，CHX不会抑制任何一个基因的转录。总的来说，这些结果表明，在正常和GCHV感染的CAB细胞中，有非常不同的机制可调节CaMx1和CaMx2的表达。

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  • 来源
    《Immunogenetics》 |2004年第1期|68-75|共8页
  • 作者

    Yi-Bing Zhang; Qiong Li; Jian-Fang Gui;

  • 作者单位

    State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences;

    State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences;

    State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences;

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  • 关键词

    Mx; Differential expression; Interferon; Antiviral-relevant genes; Innate immunity;

    机译：Mx;差异表达;干扰素;抗病毒相关基因;先天免疫;

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