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首页> 外文期刊>Indian Journal of Fibre & Textile Research >Enzymatic surface hydrolysis of polyamide fabric by protease enzyme and its production
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Enzymatic surface hydrolysis of polyamide fabric by protease enzyme and its production

机译：蛋白酶对聚酰胺织物的表面酶解及其制备

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摘要

The present study is aimed at finding out the protease enzyme specific for hydrolysis of polyamide fabric surface and its production in cost effective medium for application in textile industry. Hydrolysis of polyamide fabric by protease enzymes produced by Bacillus isolates 2W, 16P and 36P is confirmed by improved hydrophilic properties, enhanced staining with basic dyes, scanning electron microscope and FTTR. SEM images of protease treated fabric show heterogeneous appearance as cracks, pits and rough surfaces. FTIR measurements prove the increase in free amino groups on the surface of protease treated polyamide. At the same time, treated fabric shows good physical and mechanical properties. It is also observed that the loss of strength of treated fabric is 6%. Air permeability increases and static charges reduce. Bacillus isolate 16P is chosen for economic production of polyamide surface modifying protease enzyme using 14 agro-industrial by-products as growth media. The highest protease production (175 U/mL) is achieved in media containing 6% linen meal at pH 7, after 72 h incubation period. Addition of KC1 to linen meal medium enhances the protease production to about 18%.
机译：本研究的目的是找出对聚酰胺织物表面水解具有特异性的蛋白酶，并在具有成本效益的介质中生产该酶，以用于纺织工业。改进的亲水性能，增强的碱性染料染色，扫描电子显微镜和FTTR证实了芽孢杆菌分离物2W，16P和36P产生的蛋白酶水解聚酰胺织物。蛋白酶处理过的织物的SEM图像显示出异质外观，如裂纹，凹坑和粗糙表面。 FTIR测量证明蛋白酶处理的聚酰胺表面的游离氨基增加。同时，处理过的织物表现出良好的物理和机械性能。还观察到，经处理的织物的强度损失为6％。透气性增加，静电荷减少。选择芽孢杆菌分离物16P可以经济生产聚酰胺表面改性蛋白酶，它使用14种农业工业副产物作为生长介质。温育72小时后，在pH为7的含6％亚麻粉的培养基中，蛋白酶产量最高（175 U / mL）。向亚麻粉培养基中添加KC1可将蛋白酶产量提高至约18％。

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