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首页> 外文期刊>Inflammation >Tetracyclines and Chemically Modified Tetracycline-3 (CMT-3) Modulate Cytokine Secretion by Lipopolysaccharide-Stimulated Whole Blood
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Tetracyclines and Chemically Modified Tetracycline-3 (CMT-3) Modulate Cytokine Secretion by Lipopolysaccharide-Stimulated Whole Blood

机译：四环素和化学修饰的四环素-3（CMT-3）通过脂多糖刺激的全血调节细胞因子的分泌

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In addition to their bacteriostatic effect, tetracyclines, which are often used in the treatment of periodontitis, also present anti-inflammatory properties. In the present study, we investigated the effects of tetracycline (TC), doxycycline (doxy), and chemically modified tetracycline-3 (CMT-3) on the production of pro-inflammatory mediators and matrix metalloproteinases (MMPs) in an ex vivo human whole blood (WB) model stimulated with Porphyromonas gingivalis lipopolysaccharide (LPS). WB samples obtained from three periodontitis patients and six healthy subjects were stimulated with P. gingivalis LPS in the absence and presence of TC, doxy, or CMT-3. The secretion of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), MMP-8, and MMP-9 by the WB samples was determined using enzyme-linked immunosorbent assays. P. gingivalis LPS significantly increased the secretion of all cytokines and MMPs tested. While we observed inter-patient variations, TC, doxy, and CMT-3 caused reductions of LPS-induced cytokine secretion to various degrees. TC, doxy, and CMT-3 had no significant effect on MMP-8 and MMP-9 secretion by LPS-stimulated WB samples. In conclusion, we used a human WB model that takes into consideration relevant in vivo immune cell interactions in the presence of plasma proteins to show that TC, doxy, and CMT-3 can reduce the production of pro-inflammatory mediators. This property may contribute to the clinically proven benefits of these molecules in the treatment of periodontitis and other chronic inflammatory diseases.
机译：除了具有抑菌作用外，常用于治疗牙周炎的四环素还具有抗炎作用。在本研究中，我们调查了四环素（TC），强力霉素（doxy）和化学修饰的四环素3（CMT-3）对离体人类促炎性介质和基质金属蛋白酶（MMP）产生的影响牙龈卟啉单胞菌脂多糖（LPS）刺激的全血（WB）模型。在不存在和存在TC，doxy或CMT-3的情况下，用牙龈丙酸杆菌LPS刺激从三名牙周炎患者和六名健康受试者获得的WB样品。用酶联免疫吸附法测定WB样品的白介素-1β（IL-1β），白介素-6（IL-6），白介素8（IL-8），MMP-8和MMP-9的分泌。牙龈卟啉单胞菌LPS显着增加了测试的所有细胞因子和MMP的分泌。虽然我们观察到患者之间的差异，但TC，doxy和CMT-3导致LPS诱导的细胞因子分泌不同程度的降低。 TC，doxy和CMT-3对LPS刺激的WB样品的MMP-8和MMP-9分泌没有明显影响。总之，我们使用了人类WB模型，该模型考虑了血浆蛋白存在下相关的体内免疫细胞相互作用，显示TC，doxy和CMT-3可以减少促炎性介质的产生。这种性质可能有助于这些分子在牙周炎和其他慢性炎性疾病的治疗中临床证明的益处。

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