...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Inflammation >Mannitol Upregulates Monocyte HLA-DR, Monocyte and Neutrophil CD11b, and Inhibits Neutrophil Apoptosis
【24h】

Mannitol Upregulates Monocyte HLA-DR, Monocyte and Neutrophil CD11b, and Inhibits Neutrophil Apoptosis

机译：甘露醇上调单核细胞HLA-DR，单核细胞和中性粒细胞CD11b，并抑制中性粒细胞凋亡

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

d-Mannitol is a substance widely used for both clinical applications as a strong diuretic and in basic research as a purportedly inert osmotic control substance. However, recent experiments have shown that mannitol is able to decrease neutrophil apoptosis in vitro by more than 25%. The aim of the current study was to assess mannitol’s effects on two immune cell activation markers; CD11b on neutrophils and monocytes, and HLA-DR on monocytes. Exposure of diluted whole blood (1:10 in RPMI 1640) to increasing concentrations of mannitol for 24 h was associated with a significant increase in both monocyte and neutrophil CD11b expression in non-lipopolysaccharide (LPS)-stimulated samples. Monocyte HLA-DR, but not neutrophil HLA-DR, was significantly higher in the presence of 16.5 mM/l mannitol, whereas isoosmolar NaCl had no effect. Levels of IL-6 and TNF-α were not affected by mannitol in our model. All reagents were tested negative for endotoxin contamination. Together, these data indicate that mannitol may directly interact with neutrophils and monocytes. Further systematic evaluation is indicated to define the precise immunomodulating actions of mannitol, and to assess these effects in patients receiving such therapy.
机译：d-甘露醇是一种广泛用于临床应用的强利尿剂，并在基础研究中被用作惰性渗透控制质。然而，最近的实验表明，甘露醇能够在体外减少嗜中性粒细胞凋亡超过25％。本研究的目的是评估甘露醇对两种免疫细胞激活标记物的作用。中性粒细胞和单核细胞上的CD11b，单核细胞上的HLA-DR。将稀释的全血（RPMI 1640中的1:10）暴露于浓度升高的甘露醇24小时与非脂多糖（LPS）刺激的样品中单核细胞和中性粒细胞CD11b表达的显着增加有关。在存在16.5 mM / l甘露醇的情况下，单核细胞HLA-DR而非中性粒细胞HLA-DR显着更高，而等渗的NaCl则无作用。在我们的模型中，IL-6和TNF-α的水平不受甘露醇的影响。所有试剂的内毒素污染均呈阴性。这些数据一起表明甘露醇可能直接与嗜中性粒细胞和单核细胞相互作用。已表明需要进一步的系统评估，以定义甘露醇的精确免疫调节作用，并评估接受这种疗法的患者的这些作用。

著录项

  • 来源
    《Inflammation》 |2008年第2期|74-83|共10页
  • 作者

    Matthias Turina; Aaron Mulhall; Sarah Gardner; Hiram C. Polk Jr.; Frederick N. Miller;

  • 作者单位

    Department of Surgery Price Institute of Surgical Research University of Louisville School of Medicine Louisville KY USA;

    Department of Surgery Price Institute of Surgical Research University of Louisville School of Medicine Louisville KY USA;

    Department of Surgery Price Institute of Surgical Research University of Louisville School of Medicine Louisville KY USA;

    Department of Surgery Price Institute of Surgical Research University of Louisville School of Medicine Louisville KY USA;

    Department of Physiology University of Louisville Louisville KY USA;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    mannitol; neutrophil; monocyte; CD11b; HLA-DR; apoptosis;

    机译：甘露醇;嗜中性粒细胞;单核细胞;CD11b;HLA-DR;细胞凋亡;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号